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基于超高效液相色谱-四极杆-静电场轨道阱质谱法探讨黄芩汤对溃疡性结肠炎大鼠尿液代谢轮廓的影响

Effect of Huangqin Tang on Urine Metabolic Profile in Rats with Ulcerative Colitis Based on UPLC-Q-Exactive Orbitrap MS.

作者信息

Wang Dunfang, Ma Xuran, Guo Shanshan, Wang Yanli, Li Tao, Zou Dixin, Song Hongxin, Yang Weipeng, Ge Yongxiang

机构信息

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

The Experimental Research Center, China Academy of Chinese Medical Sciences, Beijing 100700, China.

出版信息

Evid Based Complement Alternat Med. 2020 Apr 22;2020:1874065. doi: 10.1155/2020/1874065. eCollection 2020.

DOI:10.1155/2020/1874065
PMID:32382278
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7193274/
Abstract

As a classic prescription, Huangqin Tang (HQT) has been widely applied to treat ulcerative colitis (UC), although its pharmacological mechanisms are not clear. In this study, urine metabolomics was first analysed to explore the therapeutic mechanisms of HQT in UC rats induced by TNBS. We identified 28 potential biomarkers affected by HQT that might cause changes in urine metabolism in UC rats, mapped the network of metabolic pathways, and revealed how HQT affects metabolism of UC rats. The results showed that UC affects amino acid metabolism and biosynthesis of unsaturated fatty acids and impairs the tricarboxylic acid cycle (TCA cycle). UC induced inflammatory and gastrointestinal reactions by inhibiting the transport of fatty acids and disrupting amino acid metabolism. HQT plays key roles via regulating the level of biomarkers in the metabolism of amino acids, lipids, and so on, normalizing metabolic disorders. In addition, histopathology and other bioinformatics analysis further confirm that HQT altered UC rat physiology and pathology, ultimately affecting metabolic function of UC rats.

摘要

作为经典方剂，黄芩汤（HQT）已被广泛应用于治疗溃疡性结肠炎（UC），但其药理机制尚不清楚。在本研究中，首次进行尿液代谢组学分析以探究黄芩汤对三硝基苯磺酸（TNBS）诱导的UC大鼠的治疗机制。我们鉴定出28种受黄芩汤影响的潜在生物标志物，这些标志物可能导致UC大鼠尿液代谢发生变化，绘制了代谢途径网络，并揭示了黄芩汤如何影响UC大鼠的代谢。结果表明，UC影响氨基酸代谢和不饱和脂肪酸的生物合成，并损害三羧酸循环（TCA循环）。UC通过抑制脂肪酸转运和扰乱氨基酸代谢诱导炎症和胃肠道反应。黄芩汤通过调节氨基酸、脂质等代谢中生物标志物的水平发挥关键作用，使代谢紊乱恢复正常。此外，组织病理学和其他生物信息学分析进一步证实黄芩汤改变了UC大鼠的生理和病理状态，最终影响UC大鼠的代谢功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/45a6d824e299/ECAM2020-1874065.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/2ac3c4ebc911/ECAM2020-1874065.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/314a6607ac9a/ECAM2020-1874065.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/7f4e852bf399/ECAM2020-1874065.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/c766c27083cd/ECAM2020-1874065.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/45a6d824e299/ECAM2020-1874065.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/2ac3c4ebc911/ECAM2020-1874065.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/a5e171697638/ECAM2020-1874065.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/38c62b68f2d8/ECAM2020-1874065.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/0b88bd4f43f0/ECAM2020-1874065.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/314a6607ac9a/ECAM2020-1874065.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/7f4e852bf399/ECAM2020-1874065.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/c766c27083cd/ECAM2020-1874065.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c7f9/7193274/45a6d824e299/ECAM2020-1874065.008.jpg

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