Sheng Jie, Liu Chang, Petrovas Sophia, Wan Yinsheng, Chen Hong-Duo, Seeram Navindra P, Ma Hang
Department of Anesthesiology, The First Hospital of China Medical University, Shenyang, China.
Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, Rhode Island, USA.
Dermatol Ther. 2020 May;33(3):e13426. doi: 10.1111/dth.13426. Epub 2020 May 3.
Reactive carbonyl species including methylglyoxal (MGO) are oxidation metabolites of glucose and precursors of advanced glycation end products (AGEs). They are important mediators of cellular oxidative stress and exacerbate skin complications. Published data supports that certain phenolic compounds can exert cellular protective effects by their antioxidant activity. A phenolic-enriched maple syrup extract (MSX) was previously reported to show protective effects against AGEs- and MGO-induced cytotoxicity in human colon cells but its skin protective effects remain unknown. The protective effects of MSX were evaluated against hydrogen peroxide (H O )- and MGO-induced cytotoxicity in human keratinocytes (HaCaT cells). Cellular viability and antioxidant activity were evaluated by the luminescent cell viability CellTiter-Glo assay and the reactive oxygen species (ROS) assay, respectively. A single-cell gel electrophoresis (Comet assay) was used to measure the strand breaks in the DNA of HaCaT cells. MSX (at 50 μg/mL) ameliorated H O - and MGO-induced cytotoxicity by increasing cell viability by 21.5% and 25.9%, respectively. MSX reduced H O - and MGO-induced ROS production by 69.4% and 56.6%, respectively. MSX also reduced MGO-induced DNA damage by 47.5%. MSX showed protective effects against H O - and MGO-induced cytotoxicity in HaCaT cells supporting its potential for dermatological and/or cosmeceutical applications.
包括甲基乙二醛（MGO）在内的活性羰基化合物是葡萄糖的氧化代谢产物和晚期糖基化终产物（AGEs）的前体。它们是细胞氧化应激的重要介质，会加剧皮肤并发症。已发表的数据表明，某些酚类化合物可通过其抗氧化活性发挥细胞保护作用。先前有报道称，富含酚类的枫糖浆提取物（MSX）对人结肠细胞中AGEs和MGO诱导的细胞毒性具有保护作用，但其皮肤保护作用尚不清楚。评估了MSX对人角质形成细胞（HaCaT细胞）中过氧化氢（H₂O₂）和MGO诱导的细胞毒性的保护作用。分别通过发光细胞活力CellTiter-Glo测定法和活性氧（ROS）测定法评估细胞活力和抗氧化活性。使用单细胞凝胶电泳（彗星试验）测量HaCaT细胞DNA中的链断裂。MSX（50μg/mL）分别使细胞活力提高21.5%和25.9%，从而改善了H₂O₂和MGO诱导的细胞毒性。MSX分别使H₂O₂和MGO诱导的ROS产生减少69.4%和56.6%。MSX还使MGO诱导的DNA损伤减少47.5%。MSX对HaCaT细胞中H₂O₂和MGO诱导的细胞毒性具有保护作用，这支持了其在皮肤病学和/或药妆应用中的潜力。
