石榴（）酚类物质改善过氧化氢诱导的人角质形成细胞氧化应激和细胞毒性。
Pomegranate () Phenolics Ameliorate Hydrogen Peroxide-Induced Oxidative Stress and Cytotoxicity in Human Keratinocytes.
作者信息
Liu Chang, Guo Hao, DaSilva Nicholas A, Li Dongli, Zhang Kun, Wan Yinsheng, Gao Xing-Hua, Chen Hong-Duo, Seeram Navindra P, Ma Hang
机构信息
Bioactive Botanical Research Laboratory, Department of Biomedical and Pharmaceutical Sciences, College of Pharmacy, University of Rhode Island, Kingston, RI 02881, USA.
Department of Dermatology, Key Laboratory of Immunodermatology, No.1 Hospital of China Medical University, Shenyang 110001, China.
出版信息
J Funct Foods. 2019 Mar;54:559-567. doi: 10.1016/j.jff.2019.02.015. Epub 2019 Feb 11.
Abstract
Pomegranate phenolics have been reported to exert skin beneficial effects but their mechanisms of action remain unclear. Herein, we investigated a standardized commercial pomegranate extract (PE; Pomella®) and its phenolics including punicalagin (PA), ellagic acid (EA), and urolithin A (UA) for their protective effects against hydrogen peroxide (HO)-induced oxidative stress and cytotoxicity in human keratinocyte HaCaT cells. PE, PA, and EA reduced the production of HO-induced ROS in HaCaT cells by 1.03-, 1.37-, and 2.67-fold, respectively. PE, PA, and UA increased the viability of HO-stimulated HaCaT cells by 89.9, 94.9, and 90.0%, respectively. PE, PA, and UA reduced apoptotic cell populations by 3.39, 7.11, and 8.26%, respectively. In addition, PE, PA and UA decreased HO-stimulated caspase-3 level by 2.31-, 2.06-, and 2.68-fold, respectively. The ameliorative effects of this PE and its phenolics against the HO-induced oxidative stress and cytotoxicity in keratinocytes support their utilization as natural cosmeceuticals for skin health. .
摘要
据报道，石榴酚类物质对皮肤有益，但其作用机制尚不清楚。在此，我们研究了一种标准化的商业石榴提取物（PE；Pomella®）及其酚类物质，包括石榴皮素（PA）、鞣花酸（EA）和尿石素A（UA）对过氧化氢（HO）诱导的人角质形成细胞HaCaT细胞氧化应激和细胞毒性的保护作用。PE、PA和EA分别使HO诱导的HaCaT细胞中ROS的产生减少了1.03倍、1.37倍和2.67倍。PE、PA和UA分别使HO刺激的HaCaT细胞活力提高了89.9%、94.9%和90.0%。PE、PA和UA分别使凋亡细胞群体减少了3.39%、7.11%和8.26%。此外，PE、PA和UA分别使HO刺激的caspase-3水平降低了2.31倍、2.06倍和2.68倍。这种PE及其酚类物质对角质形成细胞中HO诱导的氧化应激和细胞毒性的改善作用支持了它们作为皮肤健康天然化妆品的应用。
相似文献
1
Pomegranate () Phenolics Ameliorate Hydrogen Peroxide-Induced Oxidative Stress and Cytotoxicity in Human Keratinocytes.石榴（）酚类物质改善过氧化氢诱导的人角质形成细胞氧化应激和细胞毒性。
J Funct Foods. 2019 Mar;54:559-567. doi: 10.1016/j.jff.2019.02.015. Epub 2019 Feb 11.
2
Standardized Pomegranate (Pomella) and Red Maple (Maplifa) Extracts and Their Phenolics Protect Type I Collagen by the Inhibition of Matrix Metalloproteinases, Collagenase, and Collagen Cross-Linking.标准化石榴（Pomella）和红枫（Maplifa）提取物及其酚类物质通过抑制基质金属蛋白酶、胶原酶和胶原交联来保护 I 型胶原蛋白。
Molecules. 2022 Nov 16;27(22):7919. doi: 10.3390/molecules27227919.
3
Optimization of Fermentation Process of Pomegranate Peel and and the Biological Activities of Fermentation Broth: Antioxidant Activity and Protective Effect Against HO-induced Oxidative Damage in HaCaT Cells.石榴皮发酵工艺的优化及发酵液的生物活性：抗氧化活性及对 HO 诱导 HaCaT 细胞氧化损伤的保护作用。
Molecules. 2021 Jun 5;26(11):3432. doi: 10.3390/molecules26113432.
4
Pomegranate phenolics inhibit formation of advanced glycation endproducts by scavenging reactive carbonyl species.石榴酚类物质通过清除活性羰基化合物来抑制晚期糖基化终产物的形成。
Food Funct. 2014 Nov;5(11):2996-3004. doi: 10.1039/c4fo00538d.
5
Punicalagin reduces HO-induced cytotoxicity and apoptosis in PC12 cells by modulating the levels of reactive oxygen species.鞣花酸通过调节活性氧水平降低 HO 诱导的 PC12 细胞毒性和凋亡。
Nutr Neurosci. 2018 Jul;21(6):447-454. doi: 10.1080/1028415X.2017.1306935. Epub 2017 Apr 9.
6
Prospective Randomized, Double-Blind, Placebo-Controlled Study of a Standardized Oral Pomegranate Extract on the Gut Microbiome and Short-Chain Fatty Acids.标准化口服石榴提取物对肠道微生物群和短链脂肪酸的前瞻性随机、双盲、安慰剂对照研究。
Foods. 2023 Dec 19;13(1):15. doi: 10.3390/foods13010015.
7
In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice.正如石榴汁中所含的那样，与其他多酚类物质联合使用时，石榴皮素、鞣花酸和石榴总单宁提取物的体外抗增殖、凋亡和抗氧化活性会增强。
J Nutr Biochem. 2005 Jun;16(6):360-7. doi: 10.1016/j.jnutbio.2005.01.006.
8
Phenolic-enriched maple syrup extract protects human keratinocytes against hydrogen peroxide and methylglyoxal induced cytotoxicity.富含酚类的枫糖浆提取物可保护人类角质形成细胞免受过氧化氢和甲基乙二醛诱导的细胞毒性作用。
Dermatol Ther. 2020 May;33(3):e13426. doi: 10.1111/dth.13426. Epub 2020 May 3.
9
Protective effects of dissolved molecular hydrogen against hydrogen peroxide-, hydroperoxide-, and glyoxal-induced injuries to human skin keratinocytes.溶解分子氢对人皮肤角质形成细胞免受过氧化氢、过氧化物和乙二醛损伤的保护作用。
Mol Cell Biochem. 2021 Oct;476(10):3613-3622. doi: 10.1007/s11010-021-04189-z. Epub 2021 May 24.
10
Protective Effects of Minor Components of Curcuminoids on Hydrogen Peroxide-Treated Human HaCaT Keratinocytes.姜黄素类小分子成分对过氧化氢处理的人HaCaT角质形成细胞的保护作用
J Agric Food Chem. 2016 May 11;64(18):3598-608. doi: 10.1021/acs.jafc.6b01196. Epub 2016 Apr 28.
引用本文的文献
1
Preparation and characterization of immobilized mannanase on polyhydroxyalkanoate (PHA).聚羟基脂肪酸酯（PHA）固定化甘露聚糖酶的制备与表征
Bioresour Bioprocess. 2025 Jun 25;12(1):68. doi: 10.1186/s40643-025-00886-5.
2
A Proprietary Extract, Its Antioxidant Properties Using Multi-Radical Assays and Protection Against UVA-Induced Damages in a Reconstructed Human Skin Model.一种专利提取物，利用多自由基分析方法研究其抗氧化特性以及在重建人皮肤模型中对UVA诱导损伤的防护作用。
Antioxidants (Basel). 2025 Feb 28;14(3):301. doi: 10.3390/antiox14030301.
3
Neuroprotective effects of Indole 3-carbinol against Scopolamine-Induced cognitive and memory impairment in rats: modulation of oxidative stress, inflammatory and cholinergic pathways.吲哚 - 3 - 甲醇对东莨菪碱诱导的大鼠认知和记忆损伤的神经保护作用：对氧化应激、炎症和胆碱能通路的调节
Metab Brain Dis. 2025 Mar 18;40(4):154. doi: 10.1007/s11011-025-01577-y.
4
Dietary Interventions, Supplements, and Plant-Derived Compounds for Adjunct Vitiligo Management: A Review of the Literature.用于白癜风辅助治疗的饮食干预、补充剂及植物源化合物：文献综述
Nutrients. 2025 Jan 20;17(2):357. doi: 10.3390/nu17020357.
5
Anti-inflammatory and antioxidant properties of oleuropein in human keratinocytes characterized by bottom-up proteomics.通过自下而上蛋白质组学表征橄榄苦苷在人角质形成细胞中的抗炎和抗氧化特性。
Front Pharmacol. 2025 Jan 8;15:1496078. doi: 10.3389/fphar.2024.1496078. eCollection 2024.
6
Pomegranate ( L.) Extract Effects on Inflammaging.石榴（L.）提取物对炎症衰老的影响。
Molecules. 2024 Sep 3;29(17):4174. doi: 10.3390/molecules29174174.
7
Morin attenuated the global cerebral ischemia via antioxidant, anti-inflammatory, and antiapoptotic mechanisms in rats.莫林通过抗氧化、抗炎和抗细胞凋亡机制减轻了大鼠的全脑缺血。
Metab Brain Dis. 2024 Oct;39(7):1323-1334. doi: 10.1007/s11011-024-01410-y. Epub 2024 Aug 13.
8
Mediterranean Food By-products as a Valuable Source of Bioactive Compounds with Health Properties.地中海食品副产品作为具有健康特性的生物活性化合物的宝贵来源。
Curr Med Chem. 2024 Jul 25. doi: 10.2174/0109298673309549240723054831.
9
Development of a New Extraction Method for Pomegranate and Metabolite Profiling by a LC-MS and H NMR Combined Approach.一种用于石榴的新型提取方法及通过液相色谱-质谱联用和核磁共振氢谱联合方法进行代谢物谱分析的研究
Foods. 2024 May 7;13(10):1429. doi: 10.3390/foods13101429.
10
L. (Pomegranate) Extracts and Their Effects on Healthy and Diseased Skin.石榴提取物及其对健康和患病皮肤的影响。
Pharmaceutics. 2024 Mar 26;16(4):458. doi: 10.3390/pharmaceutics16040458.
本文引用的文献
1
Liquid chromatography coupled with time-of-flight tandem mass spectrometry for comprehensive phenolic characterization of pomegranate fruit and flower extracts used as ingredients in botanical dietary supplements.采用液相色谱-飞行时间串联质谱联用技术对石榴果实和花提取物进行全面的酚类成分分析，这些提取物被用作植物性膳食补充剂的成分。
J Sep Sci. 2018 Aug;41(15):3022-3033. doi: 10.1002/jssc.201800480. Epub 2018 Jun 14.
2
Punicalagin and (-)-Epigallocatechin-3-Gallate Rescue Cell Viability and Attenuate Inflammatory Responses of Human Epidermal Keratinocytes Exposed to Airborne Particulate Matter PM10.鞣花酸和（-）-表没食子儿茶素-3-没食子酸酯可挽救暴露于大气细颗粒物 PM10 中的人表皮角质形成细胞的活力并减轻其炎症反应。
Skin Pharmacol Physiol. 2018;31(3):134-143. doi: 10.1159/000487400. Epub 2018 Mar 22.
3
Evaluation of Polyphenol Anthocyanin-Enriched Extracts of Blackberry, Black Raspberry, Blueberry, Cranberry, Red Raspberry, and Strawberry for Free Radical Scavenging, Reactive Carbonyl Species Trapping, Anti-Glycation, Anti-β-Amyloid Aggregation, and Microglial Neuroprotective Effects.评价黑莓、覆盆子、蓝莓、蔓越莓、覆盆子和草莓中富含多酚的花色苷提取物的自由基清除、活性羰基物种捕获、抗糖化、抗β-淀粉样蛋白聚集和小胶质细胞神经保护作用。
Int J Mol Sci. 2018 Feb 3;19(2):461. doi: 10.3390/ijms19020461.
4
Pomegranate ellagitannin-gut microbial-derived metabolites, urolithins, inhibit neuroinflammation in vitro.石榴鞣花单宁-肠道微生物衍生代谢物，尿石素，可抑制体外神经炎症。
Nutr Neurosci. 2019 Mar;22(3):185-195. doi: 10.1080/1028415X.2017.1360558. Epub 2017 Aug 7.
5
Ethanol extract of Ilex hainanensis Merr. exhibits anti-melanoma activity by induction of G/S cell-cycle arrest and apoptosis.海南冬青乙醇提取物通过诱导G/S期细胞周期阻滞和凋亡表现出抗黑色素瘤活性。
Chin J Integr Med. 2018 Jan;24(1):47-55. doi: 10.1007/s11655-017-2544-8. Epub 2017 Jul 25.
6
Antimelanogenic Effect of Urolithin A and Urolithin B, the Colonic Metabolites of Ellagic Acid, in B16 Melanoma Cells.鞣花酸的肠道代谢物尿石素 A 和尿石素 B 对 B16 黑色素瘤细胞的抗黑色素生成作用。
J Agric Food Chem. 2017 Aug 16;65(32):6870-6876. doi: 10.1021/acs.jafc.7b02442. Epub 2017 Aug 2.
7
Urolithin A alleviates myocardial ischemia/reperfusion injury via PI3K/Akt pathway.尿石素A通过PI3K/Akt通路减轻心肌缺血/再灌注损伤。
Biochem Biophys Res Commun. 2017 May 6;486(3):774-780. doi: 10.1016/j.bbrc.2017.03.119. Epub 2017 Mar 23.
8
Neuroprotective Effects of Bioavailable Polyphenol-Derived Metabolites against Oxidative Stress-Induced Cytotoxicity in Human Neuroblastoma SH-SY5Y Cells.生物可利用的多酚衍生代谢物对氧化应激诱导的人神经母细胞瘤SH-SY5Y细胞毒性的神经保护作用。
J Agric Food Chem. 2017 Feb 1;65(4):752-758. doi: 10.1021/acs.jafc.6b04538. Epub 2016 Dec 2.
9
Multiple effects of ellagic acid on human colorectal carcinoma cells identified by gene expression profile analysis.通过基因表达谱分析鉴定出鞣花酸对人结肠癌细胞的多种作用。
Int J Oncol. 2017 Feb;50(2):613-621. doi: 10.3892/ijo.2017.3843. Epub 2017 Jan 5.
10
Effects of a Standardized Phenolic-Enriched Maple Syrup Extract on β-Amyloid Aggregation, Neuroinflammation in Microglial and Neuronal Cells, and β-Amyloid Induced Neurotoxicity in Caenorhabditis elegans.标准化富含酚类的枫糖浆提取物对β-淀粉样蛋白聚集、小胶质细胞和神经元细胞中的神经炎症以及秀丽隐杆线虫中β-淀粉样蛋白诱导的神经毒性的影响。
Neurochem Res. 2016 Nov;41(11):2836-2847. doi: 10.1007/s11064-016-1998-6. Epub 2016 Jul 14.
Zotero 插件