Vasudhevan Palanisamy, Manikandan Manoharan, Thangavel Palaniswamy, Vadivukkarasi Sasikumar
Department of Environmental Science, Periyar University, Salem, India.
Department of Biochemistry, K.S. Rangasamy College of Arts and Science, Tiruchengode, India.
J Food Biochem. 2020 Jul;44(7):e13225. doi: 10.1111/jfbc.13225. Epub 2020 Apr 17.
Cucurbita maxima, is a therapeutic plant spread all over the world. The seed of C. maxima constitutes a large amount of alkaloids, phenolic compounds, Vitamin E, and other secondary metabolites. The experiment was performed in four setups (Control, Only H O , H O + 25 mg of alkaloid, H O + 50 mg of alkaloids). The enzymatic and nonenzymatic antioxidants and lipid peroxidation (LPO) were determined to analyze the antioxidant effect of partially purified alkaloids. The results showed that partially purified alkaloids from C. maxima seed reacts effectively on the erythrocytes and leukocytes antioxidant enzyme system when compared to the hydrogen peroxide group. The present results indicate that the alkaloids present in this plant can be used as a natural antioxidant for the pharmacological purposes. PRACTICAL APPLICATIONS: The C. maxima seed constitutes rich source of alkaloids, phenolic compounds, Vitamin E, and other secondary metabolites. The results obtained revealed that the purified alkaloids from C. maxima seed acts as natural antioxidants, which enhanced the potential to scavenge H O and aids in protecting the RBC and WBC cells. The presence of 27 alkaloids of which seven major alkaloids possessing higher medicinal properties like antioxidant, antimicrobial, anti-inflammatory, antitumor, anti-aging, anti-diabetic, anticancer, anti-malarial, analgesic, nematicide, pesticide, and hemolytic activity were determined from the GC-MS analysis of the extract. Alkaloids acts as major constituent in phytotheraphy and has wide range of clinical application in humans and animals. In conclusion, alkaloids from C. maxima seed may have several properties that leading to the opening of new avenues in the natural product for the therapeutic purpose.
笋瓜是一种遍布全球的药用植物。笋瓜种子含有大量生物碱、酚类化合物、维生素E和其他次生代谢产物。实验设置了四个组（对照组、仅用H₂O、H₂O + 25毫克生物碱、H₂O + 50毫克生物碱）。通过测定酶促和非酶促抗氧化剂以及脂质过氧化（LPO）来分析部分纯化生物碱的抗氧化作用。结果表明，与过氧化氢组相比，笋瓜种子中部分纯化的生物碱对红细胞和白细胞抗氧化酶系统有显著作用。目前的结果表明，该植物中的生物碱可作为天然抗氧化剂用于药理学目的。实际应用：笋瓜种子是生物碱、酚类化合物、维生素E和其他次生代谢产物的丰富来源。所得结果表明，笋瓜种子中纯化的生物碱可作为天然抗氧化剂，增强清除H₂O的能力，并有助于保护红细胞和白细胞。通过对提取物的气相色谱 - 质谱分析，确定了27种生物碱的存在，其中七种主要生物碱具有较高的药用特性，如抗氧化、抗菌、抗炎、抗肿瘤、抗衰老、抗糖尿病、抗癌、抗疟疾、镇痛、杀线虫、杀虫和溶血活性。生物碱是植物疗法的主要成分，在人类和动物中有广泛的临床应用。总之，笋瓜种子中的生物碱可能具有多种特性，可以为天然产物治疗用途开辟新途径。
