sp. nov., a novel actinobacterium isolated from Cholistan desert soil.

A Gram-stain-positive, aerobic, catalase-positive, oxidase-negative, non-mycelium-forming, motile, rod-shaped with one polar flagellum actinobacterium, designated E918, was isolated from a desert soil collected in Cholistan desert, Pakistan. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain E918 belonged to the genus and was most closely related to CGMCC 1.15091 (97.2 % similarity). The peptidoglycan was of the A3 type and the whole-cell sugar profile was found to contain galactose. The major menaquinone was MK-9(H). The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and two unidentified glycolipids. The major fatty acids identified were anteiso-C and anteiso-C. The G+C content of the genomic DNA was 68.69 mol%. The digital DNA-DNA hybridization and average nucleotide identity values between strain E918 and CGMCC 1.15091 were 28.0 and 83.4%, respectively. On the basis of its phylogenetic, phenotypic and chemotaxonomic features, strain E918 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain of is E918 (=JCM 33392=CGMCC 1.16978).