Li Lan-Yu, Yang Zi-Wen, Asem Mipeshwaree Devi, Fang Bao-Zhu, Salam Nimaichand, Alkhalifah Dalal Hussien M, Hozzein Wael N, Nie Guo-Xing, Li Wen-Jun
College of Fisheries, Henan Normal University, Xinxiang, 453007, People's Republic of China.
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, People's Republic of China.
Antonie Van Leeuwenhoek. 2019 Mar;112(3):367-374. doi: 10.1007/s10482-018-1163-0. Epub 2018 Sep 17.
A Streptomyces isolate, designated strain SYSU D8023, was isolated from a desert sand sample collected from Gurbantunggut desert, China. The characterisation of the isolate was achieved using a polyphasic taxonomic approach. The isolate was found to be Gram-positive and aerobic. The strain was found to be able to grow at 14-50 °C, pH 6.0-9.0 and in the presence of up to 7% (w/v) NaCl. Strain SYSU D8023 contains LL-diaminopimelic acid as a cell wall diamino acid. The polar lipids were identified as diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside, an unidentified glycolipid and an unidentified phospholipid. MK-9(H) and MK-9(H) were detected as the respiratory quinones, and anteiso-C, iso-C and anteiso-C as the predominant fatty acids. Pairwise comparison of the 16S rRNA gene sequences indicated that strain SYSU D8023 has a sequence identity of 97.9% to Streptomyces barkulensis RC 1831. The DNA G + C content of strain SYSU D8023 was determined to be 70.1 mol%. Based on the analyses of the phenotypic, genotypic and phylogenetic characteristics, strain SYSU D8023 was concluded to represent a novel species of the genus Streptomyces, for which the name Streptomyces desertarenae sp. nov. is proposed. The type strain of the species is SYSU D8023 (= CGMCC 4.7455 = KCTC 49023).
从中国古尔班通古特沙漠采集的沙漠沙样中分离出一株链霉菌，命名为菌株SYSU D8023。采用多相分类方法对该分离株进行了鉴定。该分离株为革兰氏阳性菌，好氧。发现该菌株能够在14-50°C、pH 6.0-9.0以及高达7%（w/v）NaCl的条件下生长。菌株SYSU D8023含有LL-二氨基庚二酸作为细胞壁二氨基酸。极性脂类被鉴定为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未鉴定的糖脂和一种未鉴定的磷脂。检测到MK-9(H)和MK-9(H)作为呼吸醌，anteiso-C、iso-C和anteiso-C作为主要脂肪酸。16S rRNA基因序列的成对比较表明，菌株SYSU D8023与巴氏链霉菌RC 1831的序列同一性为97.9%。菌株SYSU D8023的DNA G+C含量测定为70.1 mol%。基于表型、基因型和系统发育特征的分析，得出菌株SYSU D8023代表链霉菌属的一个新物种的结论，为此提出了沙漠链霉菌新种（Streptomyces desertarenae sp. nov.）。该物种的模式菌株为SYSU D8023（=CGMCC 4.7455 = KCTC 49023）。
