Department of Animal Science, Federal University of Paraná, Curitiba, 80035-050, Brazil.
DSM, São Paulo, SP, Brazil.
BMC Vet Res. 2020 Apr 19;16(1):116. doi: 10.1186/s12917-020-02321-7.
Direct-fed microbials (DFM), such as Bacillus subtilis and Bacillus licheniformis, may improve gut functionality of the host by favouring non-pathogenic bacteria and reducing the formation of putrefactive compounds. The aim of this study was to assess the nutrient digestibility, faecal characteristics and intestinal-fermentation products in dogs fed diets with Bacillus subtilis and Bacillus licheniformis. Sixteen dogs were randomly divided into two groups. Every eight dogs were fed with the control diet or the diet with the addition of 62.5 g of DFM (B. subtilis and B. licheniformis)/ton. Diets were provided throughout a 20-day adaptation period, followed by 5 days of total faecal collection. Nutrient digestibility and the metabolisable energy of the diets, plus the dogs' faecal characteristics and intestinal fermentation products were assessed.
There were no differences in nutrient digestibility (P > 0.05). However, DFM supplementation improved faecal score and resulted in less fetid faeces (P < 0.001). DFM inclusion reduced (P < 0.05) the biogenic amines concentration: putrescine, spermidine and cadaverine, besides the concentration of phenols and quinoline.
The use of B. subtillis and B. licheniformis as DFM reduce the concentration of nitrogen fermentation products in faeces and faecal odour, but the digestibility of nutrients is not altered in dogs.
直接饲喂微生物（DFM），如枯草芽孢杆菌和地衣芽孢杆菌，通过促进非致病性细菌的生长和减少腐臭化合物的形成，可能改善宿主的肠道功能。本研究旨在评估枯草芽孢杆菌和地衣芽孢杆菌添加饲粮对犬养分消化率、粪便特性和肠道发酵产物的影响。16 只犬被随机分为两组。每组中的 8 只犬分别饲喂对照饲粮或添加 62.5gDFM（枯草芽孢杆菌和地衣芽孢杆菌）/吨的饲粮。在 20 天的适应期内提供饲粮，随后进行 5 天的总粪便收集。评估了养分消化率和饲粮代谢能，以及犬的粪便特性和肠道发酵产物。
DFM 补充对养分消化率无影响（P>0.05）。然而，DFM 补充改善了粪便评分，使粪便臭味减轻（P<0.001）。DFM 降低了（P<0.05）生物胺浓度：腐胺、亚精胺和尸胺，以及酚类和喹啉的浓度。
枯草芽孢杆菌和地衣芽孢杆菌作为 DFM 使用可降低粪便中氮发酵产物和粪便臭味的浓度，但不会改变犬对养分的消化率。
