Jammes Yves, Adjriou Nabil, Kipson Nathalie, Criado Christine, Charpin Caroline, Rebaudet Stanislas, Stavris Chloé, Guieu Régis, Fenouillet Emmanuel, Retornaz Frédérique
UMR 1263 C2VN INRA INSERM, Faculty of Medicine, Aix Marseille University, Marseille, France.
Department of Internal Medicine, European Hospital, Marseille, France.
J Transl Med. 2020 Apr 19;18(1):173. doi: 10.1186/s12967-020-02341-9.
In myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS), altered membrane excitability often occurs in exercising muscles demonstrating muscle dysfunction regardless of any psychiatric disorder. Increased oxidative stress is also present in many ME/CFS patients and could affect the membrane excitability of resting muscles.
Seventy-two patients were examined at rest, during an incremental cycling exercise and during a 10-min post-exercise recovery period. All patients had at least four criteria leading to a diagnosis of ME/CFS. To explore muscle membrane excitability, M-waves were recorded during exercise (rectus femoris (RF) muscle) and at rest (flexor digitorum longus (FDL) muscle). Two plasma markers of oxidative stress (thiobarbituric acid reactive substance (TBARS) and oxidation-reduction potential (ORP)) were measured. Plasma potassium (K) concentration was also measured at rest and at the end of exercise to explore K outflow.
Thirty-nine patients had marked M-wave alterations in both the RF and FDL muscles during and after exercise while the resting values of plasma TBARS and ORP were increased and exercise-induced K outflow was decreased. In contrast, 33 other patients with a diagnosis of ME/CFS had no M-wave alterations and had lower baseline levels of TBARS and ORP. M-wave changes were inversely proportional to TBARS and ORP levels.
Resting muscles of ME/CFS patients have altered muscle membrane excitability. However, our data reveal heterogeneity in some major biomarkers in ME/CFS patients. Measurement of ORP may help to improve the diagnosis of ME/CFS. Trial registration Ethics Committee "Ouest II" of Angers (May 17, 2019) RCB ID: number 2019-A00611-56.
在肌痛性脑脊髓炎/慢性疲劳综合征（ME/CFS）中，无论是否患有精神疾病，运动肌肉中常出现膜兴奋性改变，表现为肌肉功能障碍。许多ME/CFS患者还存在氧化应激增加的情况，这可能会影响静息肌肉的膜兴奋性。
对72例患者在静息状态、递增式自行车运动期间以及运动后10分钟恢复期进行检查。所有患者至少有四项符合ME/CFS的诊断标准。为探究肌肉膜兴奋性，在运动期间（股直肌（RF））和静息状态下（趾长屈肌（FDL））记录M波。测量两种氧化应激血浆标志物（硫代巴比妥酸反应性物质（TBARS）和氧化还原电位（ORP））。还在静息和运动结束时测量血浆钾（K）浓度，以探究钾外流情况。
39例患者在运动期间及运动后RF和FDL肌肉均有明显的M波改变，同时血浆TBARS和ORP的静息值升高，运动诱导的钾外流减少。相比之下，另外33例诊断为ME/CFS的患者没有M波改变，且TBARS和ORP的基线水平较低。M波变化与TBARS和ORP水平呈负相关。
ME/CFS患者的静息肌肉存在肌肉膜兴奋性改变。然而，我们的数据揭示了ME/CFS患者一些主要生物标志物的异质性。ORP的测量可能有助于改善ME/CFS的诊断。试验注册 昂热伦理委员会“西部二区”（2019年5月17日）RCB编号：2019 - A00611 - 56。
