Department of Molecular Diagnostic, Emam Ali Educational and Therapeutic Center, Alborz University of Medical Sciences, Karaj, Iran.
Urogenital Stem Cell Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
In Vitro Cell Dev Biol Anim. 2020 Apr;56(4):313-321. doi: 10.1007/s11626-020-00445-6. Epub 2020 Apr 19.
Bladder dysfunction is one of the most common diseases that occur for a number of reasons and the current treatment modalities do not improve much in its recovery process. Tissue engineering in the last two decades has given great hope for the treatment of these disorders. In this study, a composite nanofibrous scaffold was fabricated from chitosan, collagen, and polyvinyl-alcohol polymer blend while curcumin incorporated in scaffold fibers. The scaffold supportive functions from smooth muscle cell differentiation were studied when human-induced pluripotent stem cells were cultured on the scaffolds under differentiation medium. Biocompatibility of the fabricated scaffold increased significantly by incorporating curcumin in the scaffold fibers, where protein adsorption, cell attachment, and viability were increased in the nanofiber/curcumin group compared with the other groups. In addition, the expression level of smooth muscle cell-related genes, including alpha-smooth muscle actin (αSMA), smooth muscle 22 alpha (SM-22a), Caldesmon1, and Calponin1in the stem cells upregulated while cultured in the presence of curcumin, but this increase was significantly improved while cells cultured on the nanofibers/curcumin. In addition, αSMA protein in the cells cultured on the nanofibers/curcumin expressed significantly higher than those cells cultured on the nanofibers without curcumin. It can be concluded that smooth muscle cell differentiation of the induced pluripotent stem cells promoted by curcumin and this promotion was synergistically improved while curcumin incorporated in the nanofibers. Graphical abstract.
膀胱功能障碍是由多种原因引起的最常见疾病之一，目前的治疗方法在其恢复过程中并没有多大改善。在过去的二十年中，组织工程学为这些疾病的治疗带来了巨大的希望。在这项研究中，壳聚糖、胶原蛋白和聚乙烯醇聚合物共混物被制成了一种复合纳米纤维支架，同时姜黄素被掺入到支架纤维中。当人诱导多能干细胞在分化培养基中培养在支架上时，研究了支架对平滑肌细胞分化的支持功能。通过将姜黄素掺入支架纤维中，显著提高了支架的生物相容性，其中纳米纤维/姜黄素组的蛋白质吸附、细胞附着和活力均高于其他组。此外，在存在姜黄素的情况下培养干细胞时，平滑肌细胞相关基因的表达水平，包括α-平滑肌肌动蛋白（αSMA）、平滑肌 22α（SM-22a）、钙调蛋白 1 和钙调蛋白 1 在干细胞中上调，但当细胞在纳米纤维/姜黄素上培养时，这种上调显著提高。此外，在纳米纤维/姜黄素上培养的细胞中 αSMA 蛋白的表达明显高于在没有姜黄素的纳米纤维上培养的细胞。可以得出结论，姜黄素促进了诱导多能干细胞向平滑肌细胞的分化，而姜黄素掺入纳米纤维中则协同地提高了这种促进作用。
