慢性不可预测应激大鼠中亚麻木酚的抗抑郁样作用。
Antidepressant-like effect of flaxseed in rats exposed to chronic unpredictable stress.
机构信息
Medical Psychological Center, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha, Hunan, China.
出版信息
Brain Behav. 2020 Jun;10(6):e01626. doi: 10.1002/brb3.1626. Epub 2020 Apr 19.
INTRODUCTION
Depression is a serious mental illness. However, a significant proportion of patients with depression fail to achieve remission with antidepressant therapies. This study was conducted to explore the antidepressant-like effect of flaxseed oil and flour in an animal model with depression-like behaviors induced by chronic unpredictable stress (CUS).
METHODS
Rats were randomly divided into five groups: normal control (Sham-Sham), CUS plus saline (S-SN), CUS plus escitalopram (S-Esc), CUS plus flaxseeds oil (S-FO), and CUS plus flaxseed flour (S-FF). Behaviors were tested using sucrose preference test and forced swimming test. The serum BDNF concentration, hippocampal BDNF mRNA, and protein expression were measured by enzyme-linked immunosorbent assay, real-time PCR, and Western blot, respectively.
RESULTS
The sucrose preference rate was significantly higher in S-FO and S-FF rats than in S-SN and S-Esc rats (p < .01), and lower in S-Esc rats than in Sham-Sham rats (p < .01). The immobility time was significantly shorter in S-FO and S-FF rats than in S-SN rats (p < .01), and shorter in S-Esc rats than in S-SN rats (p < .01). Plasma BDNF concentrations were significantly lower in S-FO, S-FF, and S-Esc rats than in Sham-Sham rats (p < .01); BDNF was lower in S-FO, S-FF, and S-SN rats than in S-Esc rats. The hippocampal BDNF protein expression was significantly higher in S-Esc rats than in S-SN rats (p < .05). The hippocampal BDNF mRNA expression was significantly higher in S-Esc rats than in S-SN rats (p < .01). The BDNF gene expression in plasma and the hippocampus negatively correlated with the immobility time (p < .05), but BDNF gene expression in the hippocampus positively correlated with the sucrose preference rate (p < .05).
CONCLUSION
Flaxseed oil and flaxseed flour exert antidepressant-like effect in rats exposed to chronic stress. Flaxseed may have a therapeutic effect on depression.
简介
抑郁症是一种严重的精神疾病。然而，相当一部分抑郁症患者未能通过抗抑郁药物治疗达到缓解。本研究旨在探讨慢性不可预测应激（CUS）诱导的具有抑郁样行为的动物模型中，亚麻籽油和亚麻粉的抗抑郁作用。
方法
大鼠随机分为五组：正常对照组（Sham-Sham）、CUS 加生理盐水组（S-SN）、CUS 加依地普仑组（S-Esc）、CUS 加亚麻籽油组（S-FO）和 CUS 加亚麻粉组（S-FF）。通过蔗糖偏好试验和强迫游泳试验测试行为。采用酶联免疫吸附试验、实时 PCR 和 Western blot 分别测定血清脑源性神经营养因子（BDNF）浓度、海马 BDNF mRNA 和蛋白表达。
结果
S-FO 和 S-FF 大鼠的蔗糖偏好率明显高于 S-SN 和 S-Esc 大鼠（p<0.01），明显低于 S-Esc 大鼠（p<0.01）。S-FO 和 S-FF 大鼠的不动时间明显短于 S-SN 大鼠（p<0.01），明显短于 S-Esc 大鼠（p<0.01）。S-FO、S-FF 和 S-Esc 大鼠的血浆 BDNF 浓度明显低于 Sham-Sham 大鼠（p<0.01）；S-FO、S-FF 和 S-SN 大鼠的 BDNF 浓度明显低于 S-Esc 大鼠。S-Esc 大鼠的海马 BDNF 蛋白表达明显高于 S-SN 大鼠（p<0.05）。S-Esc 大鼠的海马 BDNF mRNA 表达明显高于 S-SN 大鼠（p<0.01）。血浆和海马的 BDNF 基因表达与不动时间呈负相关（p<0.05），但海马的 BDNF 基因表达与蔗糖偏好率呈正相关（p<0.05）。
结论
亚麻籽油和亚麻粉对慢性应激大鼠具有抗抑郁作用。亚麻可能对抑郁症有治疗作用。
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