Ahmed Hassan Elham, El-Din Abd El-Rehim Abeer Sharaf, Mohammed Kholef Emad Farah, Abd-Elgwad Elsewify Wael
Department of Gastroenterology and Tropical Medicine, Faculty of Medicine, Assiut University, Assiut, Egypt.
Department of Clinical pathology, Faculty of Medicine, Aswan University, Aswan, Egypt.
Gastroenterol Hepatol Bed Bench. 2020 Spring;13(2):147-154.
This study aimed to investigate whether plasma miR-21 and miR-92a levels may be used to differentiate between patients with irritable bowel syndrome (IBS), ulcerative colitis (UC), and colorectal cancer (CRC).
miRNA expression profiles are well characterized in CRC, but these expression profiles in UC and IBS remain promising. Screening of high-risk individuals for these diseases has substantial clinical benefits.
This was a case-control study. We quantified plasma miR-21 and miR-92a expression levels in 100 samples (37 with active UC, 33 with CRC, and 30 with IBS as well as 30 healthy controls) using real-time PCR. Their diagnostic performance for discriminating these diseases was assessed using receiver-operation characteristic curve (AUC-ROC).
The studied miRNAs were differentially expressed among all participated groups. Plasma miR-21 and miR-92a levels exhibited significant upregulation in CRC as compared to IBS, UC, and healthy subjects. Both miRNAs were upregulated in the UC group as compared to IBS and healthy subjects. ROC analysis revealed promising diagnostic performance for miR-21 and miR-92a in discriminating UC from non-UC groups (IBS and healthy subjects) with AUCs of 0.844 and 0.979 respectively. It also distinguished between CRC and UC with AUCs of 0.968 and 0.887 respectively and with reasonable sensitivities and specificities.
Circulating miR-21 and miR-92a can be exploited not only as potential noninvasive biomarkers for detection of CRC, but also for differentiation between functional and organic colorectal disorders.
本研究旨在探讨血浆miR-21和miR-92a水平是否可用于区分肠易激综合征（IBS）、溃疡性结肠炎（UC）和结直肠癌（CRC）患者。
miRNA表达谱在CRC中已得到充分表征，但在UC和IBS中的这些表达谱仍有研究价值。对这些疾病的高危个体进行筛查具有重大临床意义。
这是一项病例对照研究。我们使用实时PCR定量检测了100份样本（37例活动期UC患者、33例CRC患者、30例IBS患者以及30例健康对照）中血浆miR-21和miR-92a的表达水平。使用受试者操作特征曲线（AUC-ROC）评估它们对区分这些疾病的诊断性能。
所研究的miRNA在所有参与组中表达存在差异。与IBS、UC和健康受试者相比，CRC患者血浆miR-21和miR-92a水平显著上调。与IBS和健康受试者相比，UC组中这两种miRNA均上调。ROC分析显示，miR-21和miR-92a在区分UC与非UC组（IBS和健康受试者）方面具有良好的诊断性能，AUC分别为0.844和0.979。它还分别以0.968和0.887的AUC区分了CRC和UC，且具有合理的敏感性和特异性。
循环miR-21和miR-92a不仅可作为检测CRC的潜在非侵入性生物标志物，还可用于区分功能性和器质性结直肠疾病。
