Jahanbakhsh Sareh, Azadpour Mojgan, Tavakoli Kareshk Amir, Keyhani Amir, Mahmoudvand Hossein
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Razi Herbal Medicines Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran.
J Parasit Dis. 2016 Dec;40(4):1289-1292. doi: 10.1007/s12639-015-0670-4. Epub 2015 Mar 14.
The present study was aimed to evaluate the lethal effects of Boiss (Lamiaceae) methanolic extract against protoscoleces. Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (2.5-20 mg/mL) were used for 10-60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1 % eosin staining). Obtained results showed that extract at the concentration of 20 mg/mL after 10 min of exposure killed 100 % protoscoleces. The mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 10 mg/mL was also 100 %. Lower concentrations of extract provoked a delayed protoscolicidal activity. The findings indicated potential of methanolic extract as a natural source for the producing of new scolicidal agent for use in hydatid cyst surgery.
本研究旨在评估唇形科植物博伊斯(Boiss)甲醇提取物对原头节的致死作用。原头节从患有包虫囊肿的绵羊肝脏中无菌吸出。使用不同浓度的精油(2.5 - 20毫克/毫升)处理10 - 60分钟。通过伊红排斥试验(0.1%伊红染色)确认原头节的活力。获得的结果表明,在20毫克/毫升浓度下暴露10分钟后,提取物杀死了100%的原头节。在10毫克/毫升浓度下暴露20分钟后,原头节的平均死亡率也为100%。较低浓度的提取物引发了延迟的杀原头节活性。这些发现表明甲醇提取物有潜力作为一种天然来源,用于生产在包虫囊肿手术中使用的新型杀头节剂。