Bucur Octavian
Department of Pathology, Harvard Medical School and Beth Israel Deaconess Medical Center, Boston, MA, USA.
Institute of Biochemistry of the Romanian Academy, Bucharest, Romania.
Discoveries (Craiova). 2015 Jun 30;3(2):e46. doi: 10.15190/d.2015.38.
Many technological advances have been made in the recent years, several of them with a great potential of significantly improving the diagnostic pathology field. This article discusses three of the most promising technologies, which emerged in the last one year. Fluorescent in situ sequencing can lead to the simultaneous identification of the transcriptome-wide RNA in individual cells across a tissue sections. 3D microscopy together with advanced image analysis can be used in diagnostic pathology and will especially be useful in hard to diagnose cases where the spatial relationship of the tissue components is important. Expansion microscopy physically expands the biological specimen, and is of great interest for diagnostic pathology since the cheap conventional microscopes can be used to image a symmetrically expanded tissue. In addition, digital analysis and computational pathology are an integral part of each of these three emerging technologies, which underline their importance for the future developments in diagnostic pathology.
近年来取得了许多技术进步，其中一些具有显著改善诊断病理学领域的巨大潜力。本文讨论了过去一年中出现的三种最有前景的技术。荧光原位测序可以在整个组织切片的单个细胞中同时鉴定全转录组范围的RNA。三维显微镜与先进的图像分析一起可用于诊断病理学，尤其在难以诊断的病例中很有用，因为这些病例中组织成分的空间关系很重要。扩张显微镜对生物标本进行物理扩张，对于诊断病理学非常有吸引力，因为可以使用廉价的传统显微镜对对称扩张的组织进行成像。此外，数字分析和计算病理学是这三种新兴技术中每一种的组成部分，这突出了它们对诊断病理学未来发展的重要性。
