Bradamante S, Barchiesi E, Pilotti S, Borasi G
CNR and Dipartimento di Chimica Organica e Industriale, Università di Milano, Italy.
Magn Reson Med. 1988 Dec;8(4):440-9. doi: 10.1002/mrm.1910080407.
Surgical specimens from 22 patients with infiltrating ductal carcinoma of the breast were analyzed by high-resolution 1H NMR spectroscopy at 80 and 300 MHz. Results were compared with those obtained from 9 benign (proliferative dysplasia without atypia) breast samples. NMR spectra at 80 MHz showed well-resolved water and lipid peaks so it was possible to calculate specific T1 and T2 relaxation times. The longitudinal T1 relaxation process of the water was found to be properly represented by a monoexponential function, whereas the transverse T2 decay was well accounted for by a biexponential model. Relaxation time measurements of the water were found to be a valuable tool in breast tissue characterization, in particular (a) mean T1 values from malignant and control populations are well distinguished (P less than 0.0008); (b) mean T2 values of both fast and slow components from the two populations are statistically different (P less than 0.0001) and no overlap exists in the distributions of the second component. Three-hundred-megahertz spectra allowed a detailed analysis of the lipidic component of the sample: malignant tumors were found to present in the region 1.3-1.2 ppm a species characterized by a very slow transverse relaxation time (T2 = 200-1000 ms).
对22例乳腺浸润性导管癌患者的手术标本采用80兆赫和300兆赫的高分辨率1H核磁共振光谱进行分析。将结果与9份良性（无异型性的增殖性发育异常）乳腺样本的结果进行比较。80兆赫的核磁共振光谱显示出水峰和脂质峰分辨良好，因此可以计算特定的T1和T2弛豫时间。发现水的纵向T1弛豫过程可用单指数函数恰当表示，而横向T2衰减可用双指数模型很好地解释。发现水的弛豫时间测量是乳腺组织特征化的一种有价值的工具，特别是：(a) 恶性人群和对照人群的平均T1值有明显区别（P小于0.0008）；(b) 这两个人群的快速和慢速成分的平均T2值在统计学上有差异（P小于0.0001），且第二成分的分布没有重叠。300兆赫的光谱允许对样本的脂质成分进行详细分析：发现恶性肿瘤在1.3 - 1.2 ppm区域呈现出一种横向弛豫时间非常慢（T2 = 200 - 1000毫秒）的物质。
