Description of Salinicola corii sp. nov., a Halotolerant Bacterium Isolated from Wetsalted Hides.

A novel Gram-stain-negative, moderately halotolerant, rod-shaped bacterium, designated strain L3, was isolated from a wetsalted hide in Chengdu, China. The organism grew optimally at 30 °C, at pH 8 and with 5-10% (w/v) NaCl. The major cellular fatty acids were C, Cω7c, Cω7c and C cyclo ω8c; the predominant respiratory quinone was Q-9; the phospholipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and three unidentified phospholipids. Phylogenetic trees based on the 16S rRNA, gyrB and rpoD genes' sequences, obtained using three different algorithms, clearly revealed the isolate belonged to the genus Salinicola, and was found to be closely related to Salinicola acroporae JCM 30412, Salinicola socius CGMCC 1.12383 and Salinicola lusitanus CR50. The draft genome was approximately 4.5 Mb in size with 4486 predicted coding sequences, and the G+C content was 62.6 mol%. The maximum values of ANI and dDDH between strain L3 and the three above-mentioned type species were 89.2% and 63.8%, respectively. Differential phenotypic properties, together with the genome analysis, support the view that strain L3 represents a novel species, Salinicola corii sp. nov., with the type strain L3 (=CGMCC 1.17272=KCTC 72572).