Key Laboratory of Leather Chemistry and Engineering (Sichuan University), Ministry of Education, College of Biomass Science and Engineering, Sichuan University, Chengdu, 610065, People's Republic of China.
Curr Microbiol. 2020 Aug;77(8):1932-1938. doi: 10.1007/s00284-020-01989-2. Epub 2020 Apr 20.
A novel Gram-stain-negative, moderately halotolerant, rod-shaped bacterium, designated strain L3, was isolated from a wetsalted hide in Chengdu, China. The organism grew optimally at 30 °C, at pH 8 and with 5-10% (w/v) NaCl. The major cellular fatty acids were C, Cω7c, Cω7c and C cyclo ω8c; the predominant respiratory quinone was Q-9; the phospholipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and three unidentified phospholipids. Phylogenetic trees based on the 16S rRNA, gyrB and rpoD genes' sequences, obtained using three different algorithms, clearly revealed the isolate belonged to the genus Salinicola, and was found to be closely related to Salinicola acroporae JCM 30412, Salinicola socius CGMCC 1.12383 and Salinicola lusitanus CR50. The draft genome was approximately 4.5 Mb in size with 4486 predicted coding sequences, and the G+C content was 62.6 mol%. The maximum values of ANI and dDDH between strain L3 and the three above-mentioned type species were 89.2% and 63.8%, respectively. Differential phenotypic properties, together with the genome analysis, support the view that strain L3 represents a novel species, Salinicola corii sp. nov., with the type strain L3 (=CGMCC 1.17272=KCTC 72572).
一株新型革兰氏阴性、中度嗜盐、杆状细菌，被命名为 L3 株，从中国成都的湿盐皮中分离得到。该菌在 30°C、pH8 和 5-10%（w/v）NaCl 的条件下生长最佳。主要细胞脂肪酸为 C、Cω7c、Cω7c 和 C 环 ω8c；主要呼吸醌为 Q-9；磷脂由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油和三种未鉴定的磷脂组成。基于 16S rRNA、gyrB 和 rpoD 基因序列构建的系统发育树，使用三种不同算法，清楚地表明该分离株属于盐杆菌属，与盐杆菌属 acroporae JCM 30412、盐杆菌属 socius CGMCC 1.12383 和盐杆菌属 lusitanus CR50 密切相关。基因组草图大小约为 4.5 Mb，包含 4486 个预测编码序列，G+C 含量为 62.6 mol%。L3 株与上述三种模式种之间的 ANI 和 dDDH 的最大值分别为 89.2%和 63.8%。不同的表型特性，加上基因组分析，支持 L3 株代表一个新种，即盐杆菌 corii sp. nov.，其模式株为 L3（=CGMCC 1.17272=KCTC 72572）。
