Peterson E M, Calderone R A
Infect Immun. 1977 Mar;15(3):910-5. doi: 10.1128/iai.15.3.910-915.1977.
Normal rabbit alveolar macrophages were infected in vitro with Candida albicans. Early after infection, germ tube formation of phagocytized C. albicans was inhibited in contrast to extracellular (nonphagocytized) C. albicans. Over and 8-h period, plate counts of C. albicans incubated with alveolar macrophages revealed a decrease in colony-forming units in contrast to C. albicans alone. In addition, an assay was developed which specifically measured C. albicans [3H]leucine incorporation in the presence of alveolar macrophages. Using this assay, we observed a 71 to 93% inhibition of macromolecular synthesis in C. albicans when incubated with alveolar macrophages. Autoradiographic studies showed that the inhibition of leucine incorporation was restricted to the ingested Candida.
正常兔肺泡巨噬细胞在体外被白色念珠菌感染。感染后早期，与细胞外（未被吞噬的）白色念珠菌相比，被吞噬的白色念珠菌的芽管形成受到抑制。在8小时的时间段内，与肺泡巨噬细胞一起孵育的白色念珠菌的平板计数显示，与单独的白色念珠菌相比，菌落形成单位减少。此外，还开发了一种测定方法，该方法专门测量在肺泡巨噬细胞存在下白色念珠菌的[3H]亮氨酸掺入情况。使用该测定方法，我们观察到与肺泡巨噬细胞一起孵育时，白色念珠菌的大分子合成受到71%至93%的抑制。放射自显影研究表明，亮氨酸掺入的抑制仅限于被摄取的念珠菌。
