Lehrer R I, Ferrari L G, Patterson-Delafield J, Sorrell T
Infect Immun. 1980 Jun;28(3):1001-8. doi: 10.1128/iai.28.3.1001-1008.1980.
We tested the ability of rabbit macrophages to kill Candida albicans in vitro. Resident (unstimulated) alveolar macrophages killed 28.1 +/- 1.9% of ingested organisms in 4 h, whereas resident peritoneal macrophages killed only 15.2 +/- 1.3% (mean +/- standard error of the mean, P < 0.01). Peritoneal macrophages obtained from rabbits treated 3 weeks earlier with complete Freund adjuvant showed enhanced candidacidal activity relative to normally resident peritoneal cells (28.2 +/- 3.1%, P < 0.01). Candidacidal activity by alveolar macrophages recovered from such treated animals was slightly enhanced relative to untreated alveolar macrophages (32.9 +/- 2.3%). Candidacidal activity by peritoneal and alveolar macrophages was not decreased by several agents (cyanide, azide, sulfadiazine, and phenylbutazone) that inhibit the ability of human blood monocytes to kill C. albicans. In contrast, candidacidal activity by alveolar macrophages was greatly diminished by iodoacetate, an ineffective inhibitor of this function in human monocytes. We conclude that rabbit macrophages kill C. albicans by a fungicidal mechanism distinct from the peroxidase-H2O2 mechanism of human granulocytes and monocytes, and that the fungicidal properties of peritoneal and alveolar macrophage populations are enhanced after nonspecific stimulation with complete Freund adjuvant.
我们检测了兔巨噬细胞在体外杀死白色念珠菌的能力。驻留（未受刺激）的肺泡巨噬细胞在4小时内杀死了28.1±1.9%的摄入菌，而驻留的腹腔巨噬细胞仅杀死了15.2±1.3%（平均值±平均标准误差，P<0.01）。与正常驻留的腹腔细胞相比，3周前用完全弗氏佐剂处理的兔获得的腹腔巨噬细胞显示出增强的杀念珠菌活性（28.2±3.1%，P<0.01）。从这些处理过的动物中回收的肺泡巨噬细胞的杀念珠菌活性相对于未处理的肺泡巨噬细胞略有增强（32.9±2.3%）。腹腔和肺泡巨噬细胞的杀念珠菌活性不会因几种抑制人血单核细胞杀死白色念珠菌能力的试剂（氰化物、叠氮化物、磺胺嘧啶和保泰松）而降低。相比之下，碘乙酸可使肺泡巨噬细胞的杀念珠菌活性大大降低，碘乙酸对人单核细胞的这种功能是无效抑制剂。我们得出结论，兔巨噬细胞通过一种不同于人类粒细胞和单核细胞的过氧化物酶-H2O2机制的杀菌机制杀死白色念珠菌，并且在完全弗氏佐剂进行非特异性刺激后，腹腔和肺泡巨噬细胞群体的杀菌特性会增强。
