提取物对紫外线诱导的成纤维细胞中衰老基因、蛋白质水平及细胞凋亡的抗衰老作用。
extract's antiaging effect on aging genes, protein levels, and apoptosis in UV-induced fibroblast cells.
作者信息
Widowati Wahyu, Dani Dani, Vera Vera, Wargasetia Teresa L, Rahardja Fanny, Tih Fen, Onggowidjaja Philips, Tjokropranoto Rita, Zahiroh Fadhilah H, Azis Rizal, Priyandoko Didik, Surakusumah Wahyu, Hadiprasetyo Dhanar S
机构信息
Faculty of Medicine, Maranatha Christian University, Bandung, West Java, Indonesia.
Bimolecular and Biomedical Research Center, Aretha Medika Utama, Bandung, West Java, Indonesia.
出版信息
J Taibah Univ Med Sci. 2025 Jun 9;20(3):349-358. doi: 10.1016/j.jtumed.2025.05.005. eCollection 2025 Jun.
OBJECTIVES
Ultraviolet (UV) exposure can hasten the aging process of the skin. The use of chemicals for anti-aging has long-term adverse effects. The natural ingredients of snake fruit ( L.) are known to have bioactive properties such as polyphenols, flavonoids, chlorogenic acid, and caffeic acid which have antiaging potential. The study aims to ascertain the potential of Extract (SZE) as an antiaging agent by assay.
METHODS
The SZE compound content was analyzed by LC-MS/MS. SZE viability test on human skin fibroblast (BJ) cells was carried out using the WST assay. BJ cells were UV-induced as a cell model of premature aging. SZE 6.25, 12.5, and 25 μg/mL were administered to UV-induced BJ cells. The gene expression of and were analyzed by quantitative Real-Time PCR. Elastin (ELN), Hyaluronidase (HAse), Cyclooxigenase-2 (COX-2), 8-Hydroxydeoxyguanosine (8-OHdG), and Melatonin (MT) protein levels were analyzed by ELISA assay. The apoptosis of BJ cells was analyzed using flow cytometry. One-way ANOVA in SPSS Software was used for statistical analysis.
RESULTS
Treatment with SZE increased , , and gene expression and also decreased gene expression. SZE also increased ELN and MT levels in UV-induced BJ cells. After SZE treatment, the protein levels of HAse, COX-2, and 8-OHdG decreased compared to the positive control. SZE also succeeded in maintaining the lives of BJ cells and reducing apoptosis in BJ cells.
CONCLUSIONS
SZE has the potential to be an antiaging agent by assay.
目的
紫外线照射会加速皮肤衰老过程。使用化学物质抗衰具有长期不良影响。蛇皮果（L.）的天然成分已知具有生物活性特性，如具有抗衰潜力的多酚、黄酮类化合物、绿原酸和咖啡酸。本研究旨在通过实验确定蛇皮果提取物（SZE）作为抗衰剂的潜力。
方法
采用液相色谱 - 串联质谱法（LC - MS/MS）分析SZE的化合物含量。使用WST实验对人皮肤成纤维细胞（BJ）进行SZE活力测试。BJ细胞经紫外线诱导作为早衰细胞模型。将6.25、12.5和25μg/mL的SZE给予紫外线诱导的BJ细胞。通过定量实时聚合酶链反应分析和的基因表达。采用酶联免疫吸附测定法（ELISA）分析弹性蛋白（ELN）、透明质酸酶（HAse）、环氧化酶 - 2（COX - 2）、8 - 羟基脱氧鸟苷（8 - OHdG）和褪黑素（MT）的蛋白水平。使用流式细胞术分析BJ细胞的凋亡情况。采用SPSS软件中的单因素方差分析进行统计分析。
结果
SZE处理可增加、和的基因表达，同时降低基因表达。SZE还可提高紫外线诱导的BJ细胞中ELN和MT水平。与阳性对照相比，SZE处理后HAse、COX - 2和8 - OHdG的蛋白水平降低。SZE还成功维持了BJ细胞的活力并减少了BJ细胞的凋亡。
结论
通过实验表明SZE有潜力成为一种抗衰剂。
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