Akhtar Saqib Saleem, Amby Daniel Buchvaldt, Hegelund Josefine Nymark, Fimognari Lorenzo, Großkinsky Dominik K, Westergaard Jesper Cairo, Müller Renate, Moelbak Lars, Liu Fulai, Roitsch Thomas
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Taastrup, Denmark.
Plant Health Innovation, Chr-Hansen A/S, Hørsholm, Denmark.
Front Plant Sci. 2020 Apr 7;11:297. doi: 10.3389/fpls.2020.00297. eCollection 2020.
Increasing agricultural losses due to biotic and abiotic stresses caused by climate change challenge food security worldwide. A promising strategy to sustain crop productivity under conditions of limited water availability is the use of plant growth promoting rhizobacteria (PGPR). Here, the effects of spore forming (FMCH001) on growth and physiology of maize ( L. cv. Ronaldinho) under well-watered and drought stressed conditions were investigated. Pot experiments were conducted in the automated high-throughput phenotyping platform PhenoLab and under greenhouse conditions. Results of the PhenoLab experiments showed that plants inoculated with FMCH001 exhibited increased root dry weight (DW) and plant water use efficiency (WUE) compared to uninoculated plants. In greenhouse experiments, root and shoot DW significantly increased by more than 15% in inoculated plants compared to uninoculated control plants. Also, the WUE increased in FMCH001 plants up to 46% in both well-watered and drought stressed plants. Root and shoot activities of 11 carbohydrate and eight antioxidative enzymes were characterized in response to FMCH001 treatments. This showed a higher antioxidant activity of catalase (CAT) in roots of FMCH001 treated plants compared to uninoculated plants. The higher CAT activity was observed irrespective of the water regime. These findings show that seed coating with Gram positive spore forming could be used as biostimulants for enhancing plant WUE under both normal and drought stress conditions.
气候变化导致的生物和非生物胁迫使农业损失不断增加，这对全球粮食安全构成了挑战。在水资源有限的条件下维持作物生产力的一个有前景的策略是使用植物促生根际细菌（PGPR）。在此，研究了芽孢杆菌（FMCH001）在水分充足和干旱胁迫条件下对玉米（L. cv. Ronaldinho）生长和生理的影响。盆栽试验在自动化高通量表型分析平台PhenoLab和温室条件下进行。PhenoLab试验结果表明，与未接种的植株相比，接种FMCH001的植株根干重（DW）和植物水分利用效率（WUE）有所增加。在温室试验中，与未接种的对照植株相比，接种植株的根和地上部干重显著增加了15%以上。此外，在水分充足和干旱胁迫的植株中，FMCH001处理植株的WUE均提高了46%。针对FMCH001处理，对11种碳水化合物和8种抗氧化酶的根和地上部活性进行了表征。结果表明，与未接种的植株相比，FMCH001处理植株根中的过氧化氢酶（CAT）具有更高的抗氧化活性。无论水分状况如何，均观察到较高的CAT活性。这些发现表明，用革兰氏阳性芽孢杆菌进行种子包衣可作为生物刺激剂，在正常和干旱胁迫条件下提高植物的WUE。
