Department of Gynecology and Obstetrics, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
J Clin Lab Anal. 2020 Aug;34(8):e23340. doi: 10.1002/jcla.23340. Epub 2020 Apr 22.
This study aimed to investigate the tumor circular RNA_0018289 (circ_0018289) expression and its correlation with clinical characteristics as well as survival profiles in cervical cancer patients.
A hundred and ninety-two cervical cancer patients who received surgical resection were recruited in this prospective study. Tumor tissue and paired adjacent tissue were obtained during the surgery, in which circ_0018289 expression was detected by reverse transcription quantitative polymerase chain reaction. Disease-free survival (DFS) and overall survival (OS) were recorded.
Circ_0018289 expression was upregulated in tumor tissue compared with paired adjacent tissue (P < .001), and receiver operative characteristic curve disclosed its good value for separating tumor tissue from adjacent tissue with an area under curve of 0.907 (95% CI: 0.879-0.935). Additionally, tumor circ_0018289 expression was positively associated with tumor size (P = .009), lymph node metastasis (P = .005) and Federation International of Gynecology and Obstetrics stage (P = .005). The DFS (P = .005) and OS (P = .015) were both worse in patients with circ_0018289 high expression compared to patients with circ_0018289 low expression. Meanwhile, in patients with circ_0018289 high expression, DFS and OS were the longest in patients with high+ expression followed by patients with high++ expression, and the shortest in patients with high+++ expression. Moreover, circ_0018289 high expression could independently predict worse DFS in the total cervical cancer patients (P = .042).
Circ_0018289 could serve as a potential disease monitoring and prognostic biomarker in cervical cancer patients.
本研究旨在探讨肿瘤环状 RNA_0018289（circ_0018289）在宫颈癌患者中的表达及其与临床特征和生存状况的相关性。
本前瞻性研究纳入了 192 例接受手术切除的宫颈癌患者。在手术过程中获得肿瘤组织和配对的相邻组织，通过逆转录定量聚合酶链反应检测 circ_0018289 的表达。记录无病生存期（DFS）和总生存期（OS）。
与配对的相邻组织相比，肿瘤组织中 circ_0018289 的表达上调（P<0.001），受试者工作特征曲线表明其区分肿瘤组织和相邻组织的价值较好，曲线下面积为 0.907（95%CI：0.879-0.935）。此外，肿瘤 circ_0018289 的表达与肿瘤大小（P=0.009）、淋巴结转移（P=0.005）和国际妇产科联合会（FIGO）分期（P=0.005）呈正相关。circ_0018289 高表达患者的 DFS（P=0.005）和 OS（P=0.015）均较 circ_0018289 低表达患者差。同时，在 circ_0018289 高表达的患者中，DFS 和 OS 最长的是高+表达患者，其次是高++表达患者，最短的是高+++表达患者。此外，circ_0018289 高表达可独立预测宫颈癌患者DFS 不良（P=0.042）。
circ_0018289 可作为宫颈癌患者疾病监测和预后的潜在生物标志物。
