Murugan Nirosha J, Persinger Michael A, Karbowski Lukasz M, Dotta Blake T
Behavioural Neuroscience & Biomolecular Science, Laurentian University, 935 Ramsey Lake Road, Sudbury, ON P3E 2C6, Canada.
Department of Biology, Algoma University, Sault Ste. Marie, ON P6A 2G4, Canada.
Cancers (Basel). 2020 Apr 18;12(4):1001. doi: 10.3390/cancers12041001.
Early detection of cancer improves treatment options and increases survival. Building upon previous demonstrations that ultraweak photon emissions (UPE) could be measured to detect cancers, we designed an early detection protocol to test malignancy in both in vitro and in vivo systems. Photons were measured for 100 s from plates containing ~1 million malignant or non-malignant cells from 13 different types of human and mouse cell lines. Tumor cells displayed increased photon emissions compared to non-malignant cells. Examining the standardized Spectral Power Density (SPD) configurations for flux densities between 0.1 and 25 Hz (Δ = 0.01 Hz) yielded 90% discriminant accuracy. The emission profiles of mice that had been injected with melanoma cells could be differentiated from a non-malignant reference groups as early as 24 h post-injection. The peak SPD associated with photon emissions was ~20 Hz for both malignant cell cultures and mice with growing tumors. These results extend the original suggestion by Takeda and his colleagues (2004) published in this journal concerning the potential diagnostic value of UPEs for assessing proliferations of carcinoma cells. The specificity of the spectral profile in the 20 Hz range may be relevant to the consistent efficacy reported by several authors that weak magnetic field pulsations within this frequency range can diminish the growth of malignant cells in culture and tumor weights in mice.
癌症的早期检测可改善治疗方案并提高生存率。基于之前有关可通过测量超微弱光子发射（UPE）来检测癌症的论证，我们设计了一种早期检测方案，以在体外和体内系统中测试恶性肿瘤。从含有来自13种不同类型人类和小鼠细胞系的约100万个恶性或非恶性细胞的平板中测量光子100秒。与非恶性细胞相比，肿瘤细胞显示出光子发射增加。检查0.1至25Hz（Δ = 0.01Hz）通量密度的标准化光谱功率密度（SPD）配置，判别准确率达90%。注射黑色素瘤细胞的小鼠的发射谱在注射后24小时即可与非恶性参照组区分开来。对于恶性细胞培养物和肿瘤生长的小鼠，与光子发射相关的SPD峰值均约为20Hz。这些结果扩展了武田及其同事于2004年发表在本杂志上的关于UPE对评估癌细胞增殖的潜在诊断价值的最初建议。20Hz范围内光谱特征的特异性可能与几位作者报道的一致效果相关，即该频率范围内的弱磁场脉动可抑制培养中的恶性细胞生长和小鼠体内肿瘤重量。
