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志愿者鼻咽拭子检测呈阳性样本中 SARS-CoV-2 在精液和尿液样本中的研究。

Study of SARS-CoV-2 in semen and urine samples of a volunteer with positive naso-pharyngeal swab.

机构信息

Laboratory of Seminology-Sperm Bank "Loredana Gandini", Department of Experimental Medicine, "Sapienza" University of Rome, Viale del Policlinico 155, 00161, Rome, Italy.

Vascular and Interventional Unit, Department of Radiological Sciences, Policlinico Umberto I, "Sapienza" University of Rome, Rome, Italy.

出版信息

J Endocrinol Invest. 2020 Dec;43(12):1819-1822. doi: 10.1007/s40618-020-01261-1. Epub 2020 Apr 23.

Abstract

INTRODUCTION

The recent appearance of SARS-CoV-2 in Wuhan in 2019 has started a pandemic which has involved over a million people worldwide. A matter of debate is the possible viral detection in different body fluids than respiratory droplets. Thus, we evaluated the possible presence of SARS-CoV-2 in semen and urine samples of a volunteer with confirmed COVID-19.

MATERIALS AND METHODS

A 31-year-old man with fever, myalgia, anosmia, and ageusia was tested and found positive for SARS-CoV-2 through a pharyngeal swab. Eight days after he provided semen and urine samples in which viral RNA presence was measured using a Real time RT PCR system (RealStar SARS-CoV-2 RT-PCR, Altona Diagnostics) targeting E and S viral genes.

RESULTS AND DISCUSSION

Semen and urine samples search for SARS-CoV-2 RNA was negative. Although this should be interpreted cautiously, it may be possible that either the viral clearance kinetics in these matrices matches the progressive clinical recovery of the patient or that the virus was never present in these fluids at the time of the laboratory diagnosis.

摘要

简介

2019 年武汉出现的新型冠状病毒(SARS-CoV-2)引发了一场大流行,全球已有超过 100 万人感染。目前存在争议的是,病毒是否可能存在于呼吸道飞沫以外的其他体液中。因此,我们评估了一位确诊 COVID-19 志愿者的精液和尿液样本中是否存在 SARS-CoV-2。

材料与方法

一名 31 岁男性出现发热、肌痛、嗅觉丧失和味觉丧失,经咽拭子检测 SARS-CoV-2 呈阳性。在他提供精液和尿液样本的 8 天后,我们使用针对 E 和 S 病毒基因的 Real time RT-PCR 系统(Altona Diagnostics 的 RealStar SARS-CoV-2 RT-PCR)测量了病毒 RNA 的存在情况。

结果与讨论

精液和尿液样本中 SARS-CoV-2 RNA 的检测均为阴性。尽管这应该谨慎解释,但可能是病毒在这些基质中的清除动力学与患者的临床康复进程相匹配,或者在实验室诊断时病毒从未存在于这些体液中。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c9c5/7179792/43cd377ceba0/40618_2020_1261_Fig1_HTML.jpg

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