Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology, 35430 Gulbahce, Urla, Izmir, Turkey.
Department of Food Engineering, Faculty of Engineering, Izmir Institute of Technology, 35430 Gulbahce, Urla, Izmir, Turkey.
Bioresour Technol. 2018 Aug;261:158-165. doi: 10.1016/j.biortech.2018.03.136. Epub 2018 Apr 5.
The effect of pretreatments on the composition of the hazelnut tree pruning residue (HTPR) and on the digestibility of the cellulose was investigated. The liquid hot water (LHW) and the very dilute acid (VDA) treatments were effective in solubilizing hemicellulose. The cellulose conversion increased up to around 60% (corresponding to 32-36 g/L glucose) with decreasing hemicellulose concentration in the pretreated HTPR. The alkali treatment provided partial delignification, however, the glucose production was comparably lower. Combining the hemicellulose removal and the delignification effect of different pretreatments in two-stage processes (LHW-alkali and VDA-alkali treatments) enhanced the cellulose concentration in the solids, but not the amount of glucose released in the enzymatic digestion. These results suggested that the hemicellulose was the main barrier against the conversion of cellulose in the LHW and VDA treated HTPR and the glucose in the hydrolysis medium inhibited the cellulase activity, which prevented the complete conversion of cellulose.
研究了预处理对榛树修剪残余物（HTPR）组成和纤维素消化率的影响。液体热碱水（LHW）和非常稀酸（VDA）处理有效地溶解了半纤维素。随着预处理 HTPR 中半纤维素浓度的降低，纤维素转化率最高可达 60%左右（相当于 32-36g/L 葡萄糖）。碱处理提供了部分脱木质素，但葡萄糖产量相对较低。在两段式工艺（LHW-碱和 VDA-碱处理）中结合不同预处理的半纤维素去除和脱木质素效果，提高了纤维素在固体中的浓度，但在酶解中释放的葡萄糖量没有增加。这些结果表明，在 LHW 和 VDA 处理的 HTPR 中，半纤维素是纤维素转化的主要障碍，水解介质中的葡萄糖抑制了纤维素酶的活性，从而阻止了纤维素的完全转化。
