Henrickson K J, Kraft A J, Canter D, Shaw J
Pediatric Infectious Diseases, Children's Hospital of Wisconsin, Medical College of Wisconsin, Milwaukee, Wisconsin.
Nanogen, Inc., San Diego, California.
Clin Microbiol Newsl. 2007 Aug 1;29(15):113-119. doi: 10.1016/j.clinmicnews.2007.07.001. Epub 2007 Aug 2.
A new assay, composed of the NGEN RVA (Nanogen, Inc., San Diego, CA; Prodesse, Inc., Waukesha, WI), which is a pair of analyte-specific reagents that allow the multiplex reverse transcriptase polymerase chain reaction (RT-PCR) and electronic microarray detection of influenza virus A and B, respiratory syncytial virus A and B, and human parainfluenza virus types 1, 2, and 3, was evaluated in comparison with the Hexaplex (Prodesse), a multiplex RT-PCR-enzyme hybridization assay. Comparisons included the detection of respiratory viruses from whole-virus stocks (ATCC) and from frozen pediatric respiratory specimens collected at Children's Hospital of Wisconsin between 1991 and October 1998. After the retesting of six indeterminants and 20 discrepants, overall agreement improved to 96% on the positives and 100% on negatives, with only eight specimens still discrepant. The RVA reagents allow a rapid, sensitive, and specific assay for detecting seven of the most common respiratory viruses in children.
一种新的检测方法，由NGEN RVA（Nanogen公司，加利福尼亚州圣地亚哥；Prodesse公司，威斯康星州沃基肖）组成，它是一对分析物特异性试剂，可用于流感病毒A和B、呼吸道合胞病毒A和B以及1、2和3型人副流感病毒的多重逆转录酶聚合酶链反应（RT-PCR）和电子微阵列检测。与Hexaplex（Prodesse）这种多重RT-PCR酶杂交检测方法相比，对该新检测方法进行了评估。比较内容包括从全病毒储备液（美国典型培养物保藏中心）以及1991年至1998年10月间在威斯康星儿童医院采集的冷冻儿科呼吸道标本中检测呼吸道病毒。在对6份不确定结果和20份有差异结果进行重新检测后，阳性结果的总体一致性提高到96%，阴性结果的总体一致性达到100%，仅有8份标本仍存在差异。RVA试剂可用于快速、灵敏且特异的检测儿童中7种最常见的呼吸道病毒的检测方法。
