Beaman M, Oldfield S, MacLennan I C, Michael J, Adu D
Department of Nephrology, Queen Elizabeth Hospital, Birmingham.
Clin Exp Immunol. 1988 Dec;74(3):425-30.
The effect of the nephrotic syndrome induced by puromycin aminonucleoside (PA) in rats on specific antibody responses to 2,4 dinitrophenyl (DNP) conjugated to either spider crab haemocyanin (MSH), a T cell-dependent antigen, or hydroxyethyl starch (HES), a T cell-independent type 2 antigen were studied. The serum IgG anti-DNP levels following immunization with both antigens were reduced in nephrotic animals compared with controls while IgM anti-DNP antibody titres were higher. The half-life of IgG anti-DNP antibodies passively transferred into non-immunized nephrotic rats was markedly reduced while the half-life of anti-DNP antibodies of the IgM class was comparable to that in controls. Low serum IgG and elevated IgM levels were seen in nephrotic animals compared to controls. Antibody-forming cells specific for DNP were demonstrated by immunohistology on rat spleens and the numbers of both IgG and IgM-producing cells were found to be significantly increased (P less than 0.05) in nephrotic animals in response to both DNP-HES and DNP-MSH. These data indicate that in nephrotic rats the alteration seen in the serum immunoglobulin levels is not attributable to reduced antibody production but increased catabolism of serum IgG antibodies.
研究了嘌呤霉素氨基核苷（PA）诱导的大鼠肾病综合征对针对与蜘蛛蟹血蓝蛋白（MSH，一种T细胞依赖性抗原）或羟乙基淀粉（HES，一种T细胞非依赖性2型抗原）偶联的2,4 -二硝基苯基（DNP）的特异性抗体反应的影响。与对照组相比，肾病动物在用两种抗原免疫后血清IgG抗DNP水平降低，而IgM抗DNP抗体滴度更高。被动转移到未免疫的肾病大鼠体内的IgG抗DNP抗体的半衰期明显缩短，而IgM类抗DNP抗体的半衰期与对照组相当。与对照组相比，肾病动物血清中IgG水平低而IgM水平升高。通过对大鼠脾脏进行免疫组织学检测，发现针对DNP的抗体形成细胞，并且在肾病动物中，针对DNP - HES和DNP - MSH的IgG和IgM产生细胞的数量均显著增加（P小于0.05）。这些数据表明，在肾病大鼠中，血清免疫球蛋白水平的变化并非归因于抗体产生减少，而是血清IgG抗体分解代谢增加。
