Bernard A, McGrath J J, Fuller B J, Imoedemhe D, Shaw R W
Academic Department of Obstetrics & Gynecology, Royal Free Hospital School of Medicine, London.
Cryobiology. 1988 Dec;25(6):495-501. doi: 10.1016/0011-2240(88)90295-7.
The hydraulic conductivity (Lp) of the plasma membrane determines how cells respond to the stresses of dehydration encountered during cryopreservation. We have used a microscope diffusion chamber which allows for direct real-time observation of the dynamic osmotic response of individual cells in microvolume suspension to compare the Lp of murine and human unfertilized ova. In this system, the response of an individual cell to the induced osmotic imbalance is documented via a series of photomicrographs or videotape; from these data the Lp can be computed. Donated human preovulatory oocytes were compared with macroscopically normal human ova, 43 hr after insemination, which had failed to fertilize (Ff) and with murine ova collected 13 hr post human chorionic gonadotropin injection. The permeability coefficients were 0.65 +/- 0.43, 0.84 +/- 0.39, and 0.36 +/- 0.07 micron3/micron2/atm/min. The results suggest that it may be possible to use Ff ova for experiments to design suitable cryopreservation procedures.
质膜的水力传导率（Lp）决定了细胞在冷冻保存过程中对脱水应激的反应方式。我们使用了一种显微镜扩散室，它能够对微量悬浮液中的单个细胞的动态渗透反应进行直接实时观察，以比较小鼠和人类未受精卵的Lp。在这个系统中，通过一系列显微照片或录像记录单个细胞对诱导的渗透失衡的反应；根据这些数据可以计算出Lp。将捐赠的人类排卵前卵母细胞与授精43小时后未能受精（Ff）的外观正常的人类卵子以及在人绒毛膜促性腺激素注射13小时后采集的小鼠卵子进行比较。渗透系数分别为0.65±0.43、0.84±0.39和0.36±0.07立方微米/平方微米/大气压/分钟。结果表明，有可能将Ff卵子用于设计合适冷冻保存程序的实验。
