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血小板裂解液激活的人关节软骨祖细胞作为未来软骨工程和修复策略的工具。

Progenitor Cells Activated by Platelet Lysate in Human Articular Cartilage as a Tool for Future Cartilage Engineering and Reparative Strategies.

机构信息

Regenerative Medicine Laboratory, Department of Experimental Medicine (DIMES), University of Genova, via Leon Battista Alberti 2, 16132 Genova, Italy.

Neurobiology of miRNA, Fondazione Istituto Italiano di Tecnologia, 16163 Genova, Italy.

出版信息

Cells. 2020 Apr 23;9(4):1052. doi: 10.3390/cells9041052.

DOI:10.3390/cells9041052
PMID:32340136
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7226425/
Abstract

Regenerative strategies for human articular cartilage are still challenging despite the presence of resident progenitor cell population. Today, many efforts in the field of regenerative medicine focus on the use of platelet derivatives due to their ability to reactivate endogenous mechanisms supporting tissue repair. While their use in orthopedics continues, mechanisms of action and efficacy need further characterization. We describe that the platelet lysate (PL) is able to activate chondro-progenitor cells in a terminally differentiated cartilage tissue. Primary cultures of human articular chondrocytes (ACs) and cartilage explants were set up from donor hip joint biopsies and were treated in vitro with PL. PL recruited a chondro-progenitors (CPCs)-enriched population from ex vivo cartilage culture, that showed high proliferation rate, clonogenicity and nestin expression. CPCs were positive for in vitro tri-lineage differentiation and formed hyaline cartilage-like tissue in vivo without hypertrophic fate. Moreover, the secretory profile of CPCs was analyzed, together with their migratory capabilities. Some CPC-features were also induced in PL-treated ACs compared to fetal bovine serum (FBS)-control ACs. PL treatment of human articular cartilage activates a stem cell niche responsive to injury. These facts can improve the PL therapeutic efficacy in cartilage applications.

摘要

尽管存在常驻祖细胞群体，但人类关节软骨的再生策略仍然具有挑战性。如今，再生医学领域的许多研究都集中在使用血小板衍生制品上，因为它们能够重新激活支持组织修复的内源性机制。尽管它们在骨科中的应用仍在继续，但作用机制和疗效仍需进一步表征。我们描述了血小板裂解物 (PL) 能够在终末分化的软骨组织中激活软骨祖细胞 (CPCs)。从供体髋关节活检中建立了原代人关节软骨细胞 (AC) 和软骨外植体培养物，并在体外用 PL 进行处理。PL 从体外软骨培养物中募集了富含 CPCs 的群体，该群体表现出高增殖率、克隆形成能力和巢蛋白表达。CPCs 在体外呈三系分化阳性，并在体内形成透明软骨样组织，而无肥大命运。此外，还分析了 CPCs 的分泌谱及其迁移能力。与胎牛血清 (FBS) 对照 AC 相比，PL 处理的 AC 中也诱导了一些 CPC 特征。PL 处理人关节软骨可激活对损伤有反应的干细胞龛。这些事实可以提高 PL 在软骨应用中的治疗效果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/9a687846152e/cells-09-01052-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/a003c8da015c/cells-09-01052-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/7144ffb1cf29/cells-09-01052-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/61d9792a95f3/cells-09-01052-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/830a4493f886/cells-09-01052-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/c0713d2a68fc/cells-09-01052-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/e27d4f1b3ee5/cells-09-01052-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/9a687846152e/cells-09-01052-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/a003c8da015c/cells-09-01052-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/7144ffb1cf29/cells-09-01052-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/61d9792a95f3/cells-09-01052-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/830a4493f886/cells-09-01052-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/c0713d2a68fc/cells-09-01052-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/e27d4f1b3ee5/cells-09-01052-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86bf/7226425/9a687846152e/cells-09-01052-g007.jpg

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