用于鉴定临床标本中病毒DNA的时间分辨荧光测定法。
Time-resolved fluorometry for the identification of viral DNA in clinical specimens.
作者信息
Dahlén P, Hurskainen P, Lövgren T, Hyypiä T
机构信息
Department of Virology, University of Turku, Finland.
出版信息
J Clin Microbiol. 1988 Nov;26(11):2434-6. doi: 10.1128/jcm.26.11.2434-2436.1988.
Abstract
Time-resolved fluorometry was used for the detection of DNA probes labeled directly with europium (Eu3+) chelate. The sensitivity of this nonisotopic hybridization assay was 100 pg of homologous DNA. Equivalent results with reference tests were obtained in the detection of adenoviruses in clinical specimens.
摘要
时间分辨荧光分析法用于检测直接用铕（Eu3+）螯合物标记的DNA探针。这种非同位素杂交检测法的灵敏度为100 pg同源DNA。在检测临床标本中的腺病毒时，获得了与参考检测相当的结果。
相似文献
1
Time-resolved fluorometry for the identification of viral DNA in clinical specimens.用于鉴定临床标本中病毒DNA的时间分辨荧光测定法。
J Clin Microbiol. 1988 Nov;26(11):2434-6. doi: 10.1128/jcm.26.11.2434-2436.1988.
2
Detection of adenovirus in clinical specimens by polymerase chain reaction and liquid-phase hybridization quantitated by time-resolved fluorometry.通过聚合酶链反应及时间分辨荧光法定量液相杂交检测临床标本中的腺病毒。
J Clin Microbiol. 1993 Jul;31(7):1886-91. doi: 10.1128/jcm.31.7.1886-1891.1993.
3
Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry.使用铕标记的链霉亲和素和时间分辨荧光法检测生物素化的DNA探针。
Anal Biochem. 1987 Jul;164(1):78-83. doi: 10.1016/0003-2697(87)90370-8.
4
Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes.用放射性和非放射性DNA探针检测鼻咽标本中的腺病毒。
J Clin Microbiol. 1985 May;21(5):730-3. doi: 10.1128/jcm.21.5.730-733.1985.
5
DNA hybridization for diagnosis of enteric adenovirus infection from directly spotted human fecal specimens.通过直接点样的人类粪便标本进行DNA杂交以诊断肠道腺病毒感染。
J Clin Microbiol. 1987 Oct;25(10):1881-5. doi: 10.1128/jcm.25.10.1881-1885.1987.
6
Detection of adenoviruses in stool specimens by nucleic acid spot hybridization.通过核酸斑点杂交法检测粪便标本中的腺病毒。
J Med Virol. 1985 Jul;16(3):213-8. doi: 10.1002/jmv.1890160302.
7
Detection of bovine adenovirus nucleic acid sequences in nasal specimens by biotinylated DNA probes.利用生物素化DNA探针检测鼻标本中的牛腺病毒核酸序列。
Zentralbl Veterinarmed B. 1992 Jun;39(4):311-6. doi: 10.1111/j.1439-0450.1992.tb01175.x.
8
The use of europium (Eu3+) labelled primers in PCR amplification of specific target DNA.铕（Eu3+）标记引物在特定靶DNA的PCR扩增中的应用。
Mol Cell Probes. 1991 Apr;5(2):143-9. doi: 10.1016/0890-8508(91)90009-9.
9
Time-resolved fluorometry: a sensitive method to quantify DNA-hybrids.时间分辨荧光分析法：一种定量检测DNA杂交体的灵敏方法。
Nucleic Acids Res. 1986 Jan 24;14(2):1017-28. doi: 10.1093/nar/14.2.1017.
10
Direct detection and differentiation of fastidious and nonfastidious adenoviruses in stools by using a specific nonradioactive probe.使用特异性非放射性探针直接检测和区分粪便中苛养型和非苛养型腺病毒。
J Clin Microbiol. 1986 Nov;24(5):785-9. doi: 10.1128/jcm.24.5.785-789.1986.
引用本文的文献
1
Time-resolved fluorometry PCR assay for rapid detection of herpes simplex virus in cerebrospinal fluid.用于快速检测脑脊液中单纯疱疹病毒的时间分辨荧光定量聚合酶链反应检测法
J Clin Microbiol. 2000 Sep;38(9):3214-8. doi: 10.1128/JCM.38.9.3214-3218.2000.
2
Detection of adenovirus in clinical specimens by polymerase chain reaction and liquid-phase hybridization quantitated by time-resolved fluorometry.通过聚合酶链反应及时间分辨荧光法定量液相杂交检测临床标本中的腺病毒。
J Clin Microbiol. 1993 Jul;31(7):1886-91. doi: 10.1128/jcm.31.7.1886-1891.1993.
3
Detection of human immunodeficiency virus type 1 by using the polymerase chain reaction and a time-resolved fluorescence-based hybridization assay.利用聚合酶链反应和基于时间分辨荧光的杂交分析法检测1型人类免疫缺陷病毒
J Clin Microbiol. 1991 Apr;29(4):798-804. doi: 10.1128/jcm.29.4.798-804.1991.
4
Detection of human immunodeficiency virus type 1 RNA in plasma samples from high-risk pediatric patients by using the self-sustained sequence replication reaction.通过使用自我维持序列复制反应检测高危儿科患者血浆样本中的1型人类免疫缺陷病毒RNA。
J Clin Microbiol. 1992 Feb;30(2):281-6. doi: 10.1128/jcm.30.2.281-286.1992.
本文引用的文献
1
Chemically modified nucleic acids as immunodetectable probes in hybridization experiments.化学修饰的核酸作为杂交实验中可免疫检测的探针。
Proc Natl Acad Sci U S A. 1984 Jun;81(11):3466-70. doi: 10.1073/pnas.81.11.3466.
2
3
A colorimetric method for DNA hybridization.一种用于DNA杂交的比色法。
Nucleic Acids Res. 1984 Apr 25;12(8):3435-44. doi: 10.1093/nar/12.8.3435.
4
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes.生物素标记多核苷酸的酶促合成：新型核酸亲和探针。
Proc Natl Acad Sci U S A. 1981 Nov;78(11):6633-7. doi: 10.1073/pnas.78.11.6633.
5
Rapid and sensitive colorimetric method for visualizing biotin-labeled DNA probes hybridized to DNA or RNA immobilized on nitrocellulose: Bio-blots.用于可视化与固定在硝酸纤维素膜上的DNA或RNA杂交的生物素标记DNA探针的快速灵敏比色法：生物印迹法。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):4045-9. doi: 10.1073/pnas.80.13.4045.
6
A new method for fluorescence microscopical localization of specific DNA sequences by in situ hybridization of fluorochromelabelled RNA.一种通过荧光素标记RNA原位杂交对特定DNA序列进行荧光显微镜定位的新方法。
Exp Cell Res. 1980 Aug;128(2):485-90. doi: 10.1016/0014-4827(80)90087-7.
7
Novel test for rapid viral diagnosis: detection of adenovirus in nasopharyngeal mucus aspirates by means of nucleic-acid sandwich hybridisation.
Lancet. 1983 Feb 19;1(8321):381-3. doi: 10.1016/s0140-6736(83)91500-3.
8
Detection of adenovirus in nasopharyngeal specimens by radioactive and nonradioactive DNA probes.用放射性和非放射性DNA探针检测鼻咽标本中的腺病毒。
J Clin Microbiol. 1985 May;21(5):730-3. doi: 10.1128/jcm.21.5.730-733.1985.
9
Time-resolved fluorometry: a sensitive method to quantify DNA-hybrids.时间分辨荧光分析法：一种定量检测DNA杂交体的灵敏方法。
Nucleic Acids Res. 1986 Jan 24;14(2):1017-28. doi: 10.1093/nar/14.2.1017.
10
Non-radioactive nucleic acid probes for the diagnosis of virus infections.用于病毒感染诊断的非放射性核酸探针。
Bioessays. 1986 Mar;4(3):110-3. doi: 10.1002/bies.950040305.
Zotero 插件