酵母中由C18脂肪酸形成生物素前体庚二酸。

Formation of a biotin precursor, pimelic acid, in yeasts from C18 fatty acids.

作者信息

Ohsugi M, Miyauchi K, Tachibana K, Nakao S

机构信息

Department of Food Science, Faculty of Home Economics, Mukogawa Women's University, Nishinomiya, Japan.

出版信息

J Nutr Sci Vitaminol (Tokyo). 1988 Aug;34(4):343-52. doi: 10.3177/jnsv.34.343.

DOI:10.3177/jnsv.34.343

PMID:3236079

Abstract

The biosynthesis of biotin-vitamers from C18 fatty acids in the resting cell reaction system of yeasts was investigated. The formation of pimelic acid (a biotin precursor) and biotin-vitamers (a mixture of 7-keto-8-aminopelargonic acid and dethiobiotin) from linolenic, linoleic, and oleic acids was observed in certain yeasts belonging to the genera Cryptococcus, Candida, Rhodotorula, and Trichosporon. Metabolites from linolenic acid were analyzed by gas-liquid chromatography and mass spectrometry. Linoleic, oleic, stearic, heptadecanoic, palmitic, pentadecanoic, and pimelic acids were identified as metabolites of linolenic acid. The existence of a route to pimelic acid from linolenic acid in a strain of Rhodotorula rubra AKU 4836 was also indicated.

摘要

研究了酵母静止细胞反应系统中由C18脂肪酸生物合成生物素维生素的过程。在属于隐球菌属、念珠菌属、红酵母属和丝孢酵母属的某些酵母中，观察到了从亚麻酸、亚油酸和油酸形成庚二酸（一种生物素前体）和生物素维生素（7-酮-8-氨基壬酸和脱硫生物素的混合物）。通过气液色谱法和质谱法分析了亚麻酸的代谢产物。亚油酸、油酸、硬脂酸、十七烷酸、棕榈酸、十五烷酸和庚二酸被鉴定为亚麻酸的代谢产物。还表明在深红酵母AKU 4836菌株中存在从亚麻酸到庚二酸的途径。