Laboratory of Metabolic Engineering and Bioprocesses, Department of Food Engineering and Technology, School of Food Engineering, University of Campinas (UNICAMP) - University City Zeferino Vaz, Rua Monteiro Lobato, N° 80, ZIP Code:13.083-862, Campinas, São Paulo, Brazil.
Cereal, Roots and Tubers Laboratory, Department of Food Engineering and Technology, Faculty of Food Engineering, School of Food Engineering, University of Campinas (UNICAMP) - University City Zeferino Vaz, Rua Monteiro Lobato, N° 80, ZIP Code:13.083-862, Campinas, São Paulo, Brazil.
Food Res Int. 2024 Sep;192:114768. doi: 10.1016/j.foodres.2024.114768. Epub 2024 Jul 16.
In the context of biorefinery, researchers have been looking for lignocellulosic biomasses and ideal treatments to produce economically viable biofuels. In this scenario, the bamboo culm appears as a plant matrix of great potential, given the high cellulose content of low crystallinity. Thus, the objective and differential of this work was to determine the best conditions for enzymatic hydrolysis of cellulose extracted from bamboo culm and to evaluate its potential application in the production of bioethanol through Separate Hydrolysis and Fermentation (SHF) and Saccharification and Simultaneous Fermentation (SSF) by Saccharomyces cerevisiae modified via CRISPR/Cas9. The average cellulose extraction yield was 41.87 % with an extraction efficiency of 86.76 %. In general, as the hydrolysis time increased, an increase in glucose production was observed in almost all assays, with higher hydrolysis efficiency values at 72 h. The results ranged from 2.09 to 19.8 g/L of glucose obtained with efficiency values of 10.47 to 99 %. The best conditions were found in test 5 (temperature of 36 °C and pH 5.0, with only 10 FPU/g of substrate Cellic Ctec2 Novozymes ® cocktail). It is observed that for all hydrolysis times the independent variables pH and temperature were significant under the hydrolysis efficiency, showing a negative effect, indicating that higher values of the same promote lower values of the response variable. For bioethanol production, a maximum concentration of 7.84 g/L was observed for the SSH process after 4 h of fermentation, while for the SSF process it was 12.6 g/L after 24 h of fermentation, indicating the large potential of the simultaneous process together with the application of bamboo culm biomass for high production of biofuel.
在生物炼制的背景下，研究人员一直在寻找木质纤维素生物质和理想的处理方法来生产经济可行的生物燃料。在这种情况下，竹秆作为一种具有巨大潜力的植物基质出现，因为它的纤维素含量高且结晶度低。因此，这项工作的目的和重点是确定从竹秆中提取纤维素的最佳酶解条件，并评估其通过 CRISPR/Cas9 修饰的酿酒酵母的单独水解和发酵 (SHF) 和糖化和同时发酵 (SSF) 生产生物乙醇的潜在应用。平均纤维素提取率为 41.87%，提取效率为 86.76%。一般来说，随着水解时间的增加，几乎所有试验中都观察到葡萄糖产量的增加，在 72 小时时水解效率值更高。结果范围从 2.09 到 19.8g/L 的葡萄糖，效率值从 10.47 到 99%不等。在测试 5 中找到了最佳条件（温度为 36°C，pH 为 5.0，底物纤维素酶 Celic Ctec2 Novozymes ® 仅用 10 FPU/g）。可以看出，对于所有水解时间，水解效率下的 pH 和温度这两个自变量都是显著的，表现出负效应，表明相同变量的较高值会促进响应变量的较低值。对于生物乙醇生产，在 4 小时发酵后，SSH 工艺的最大浓度为 7.84g/L，而在 24 小时发酵后，SSF 工艺的最大浓度为 12.6g/L，这表明了同时工艺与竹秆生物量的结合具有很大的生产生物燃料的潜力。
