Graduate School of Science, Nagoya University, Nagoya, Japan.
Chem Commun (Camb). 2020 Jun 11;56(46):6217-6220. doi: 10.1039/d0cc02140g. Epub 2020 May 5.
Circular RNA without a stop codon enables rolling circle translation. To produce circular RNAs, we carried out one-pot chemical synthesis of circular RNA from RNA fragments with the use of an EDC/HOBt-based chemical ligation reaction. The synthesized circular RNAs acted as translation templates, despite the presence of unnatural phosphoramidate linkages.
无终止密码子的环状 RNA 可实现滚环翻译。为了产生环状 RNA，我们使用基于 EDC/HOBt 的化学连接反应，从 RNA 片段一锅化学合成环状 RNA。尽管存在非天然的磷酰胺键，但合成的环状 RNA 可作为翻译模板。
