Department of Medical Chemistry, Medical University of Bialystok, Adama Mickiewicza 2A street, 15-222 Bialystok, Poland.
Department of Medicinal Chemistry, Medical University of Bialystok, Adama Mickiewicza 2D street, 15-222 Bialystok, Poland.
Nutrients. 2020 May 1;12(5):1282. doi: 10.3390/nu12051282.
Parabens, which are widely used in food, medicines and cosmetics, have a harmful effect on human health. People are most exposed to parabens transdermally by using cosmetic products containing these preservatives. The purpose of this study was to estimate the influence of parabens (methylparaben-MP and propylparaben-PP) on the metabolism of collagen in the human skin fibroblasts and above all, to assess whether rosmarinic acid (RA-50, 100, or 150 M) can protect these cells from the adverse effects of parabens (0.001% MP and 0.0003% PP, 0.003% MP and 0.001% PP, and 0.01% MP and 0.003% PP). The possible mechanisms of RA action were estimated as well. Parabens decreased the expression of collagen type I and III at mRNA and protein levels, while RA (depending on the concentration) provided partial or total protection against these changes. The effective protection against the adverse effects of parabens on cell viability and proliferation was also provided by RA. The beneficial impact of RA on collagen and the fibroblasts resulted from an independent action of this compound and its interaction with parabens. This study allows us to conclude that this polyphenolic compound may protect from unfavorable health outcomes caused by lifetime human exposure to parabens contained in cosmetic products.
防腐剂被广泛应用于食品、药品和化妆品中，对人类健康有害。人们通过使用含有这些防腐剂的化妆品，经皮肤接触而最易受到防腐剂的影响。本研究旨在评估防腐剂（对羟基苯甲酸甲酯-MP 和对羟基苯甲酸丙酯-PP）对人皮肤成纤维细胞中胶原蛋白代谢的影响，尤其评估迷迭香酸（RA-50、100 或 150μM）是否能保护这些细胞免受防腐剂（0.001%MP 和 0.0003%PP、0.003%MP 和 0.001%PP 以及 0.01%MP 和 0.003%PP）的不良影响。同时还评估了 RA 作用的可能机制。防腐剂在 mRNA 和蛋白质水平上降低了 I 型和 III 型胶原蛋白的表达，而 RA（取决于浓度）对这些变化提供了部分或完全的保护。RA 还能有效防止防腐剂对细胞活力和增殖的不良影响。RA 对胶原蛋白和成纤维细胞的有益影响来自于该化合物的独立作用及其与防腐剂的相互作用。本研究可以得出结论，这种多酚化合物可能保护人类因终生接触化妆品中含有的防腐剂而导致的不良健康后果。
