Department of Medical Chemistry, Medical University of Bialystok, Mickiewicza 2A, 15-222 Bialystok, Poland.
Int J Mol Sci. 2021 Oct 23;22(21):11451. doi: 10.3390/ijms222111451.
Benzophenone-3 (BP-3) is one of the most widely used chemical sunscreens. The results of many in vitro and in vivo tests confirm its high percutaneous penetration and systemic absorption, which question the safety of its wide use. The aim of our research was to assess the effect of this compound on components of the skin extracellular matrix, and to investigate whether rosmarinic acid (RA) could reduce BP-3-induced changes in human skin fibroblasts. BP-3 used at concentrations of 0.1-100 µM caused a number of unfavorable changes in the level of type I collagen, decorin, sulfated glycosaminoglycans, hyaluronic acid, elastin, and expression or activity of matrix metalloproteinases (MMP-1, MMP-2), elastase and hyaluronidase. Moreover, the intracellular retention of collagen was accompanied by changes in the expression of proteins modifying and controlling the synthesis and secretion of this protein. Most importantly, RA at a concentration of 100 µM significantly reduced or completely abolished the adverse effects of BP-3. Based on these findings, it can be concluded that this polyphenol may provide effective protection against BP-3-induced disturbances in skin cells, which may have important clinical implications.
二苯甲酮-3（BP-3）是最广泛使用的化学防晒霜之一。许多体外和体内试验的结果证实了其高经皮渗透性和全身吸收率，这对其广泛使用的安全性提出了质疑。我们的研究目的是评估该化合物对皮肤细胞外基质成分的影响，并研究迷迭香酸（RA）是否可以减少 BP-3 对人皮肤成纤维细胞的诱导变化。BP-3 在 0.1-100 µM 的浓度下使用会引起 I 型胶原蛋白、饰胶蛋白聚糖、硫酸化糖胺聚糖、透明质酸、弹性蛋白、基质金属蛋白酶（MMP-1、MMP-2）、弹性蛋白酶和透明质酸酶的水平发生许多不利变化。此外，细胞内胶原蛋白的保留伴随着改变修饰和控制这种蛋白质合成和分泌的蛋白质的表达。最重要的是，浓度为 100 µM 的 RA 显著降低或完全消除了 BP-3 的不利影响。基于这些发现，可以得出结论，这种多酚可能为预防 BP-3 引起的皮肤细胞紊乱提供有效保护，这可能具有重要的临床意义。
