Czyżewska-Dors Ewelina, Núñez José I, Saporiti Viviane, Huerta Eva, Riutord Carme, Cabezón Oscar, Segalés Joaquim, Sibila Marina
Department of Swine Diseases, National Veterinary Research Institute, 24-100 Puławy, Poland.
IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Campus de la Universitat, Autònoma de Barcelona, 08193 Bellaterra, Spain.
Pathogens. 2020 May 1;9(5):341. doi: 10.3390/pathogens9050341.
Porcine circovirus 3 (PCV-3) is the third member of the family , genus , able to infect swine. A high prevalence of viral DNA has been recorded in wild boars. Recently, PCV-3 DNA was identified in Italian wild ruminants. Based on these previous results, this study assessed the frequency of PCV-3 DNA detection in free-ranging ruminants and species in Spain. In addition, the genetic characterization of the PCV-3 PCR-positive samples was performed. A total of 801 serum samples, including red deer (; n = 108), roe deer (; n = 87), Pyrenean chamois (; n = 133), Iberian ibex (; n = 92), mouflon (; n = 91), fallow deer (; n = 104), European rabbit (; n = 101), and European hare (; n = 85) from Catalonia (northeast Spain) were tested by conventional polymerase chain reaction (PCR) and, when positive, sequenced. Overall, PCV-3 DNA was found in three out of 801 analyzed sera (0.37%) corresponding to one red deer (1/108, 0.9%), one mouflon (1/91, 1.1%), and one fallow deer (1/104, 0.96%). None of the samples collected from species resulted PCR positive. The partial genome sequences detected in positive samples displayed high identity with some PCV-3 sequences detected in wild boars and domestic pigs (99.7% and 100%, respectively). In conclusion, the present study indicated that free-ranging ruminant and species are not relevant in the epidemiology of PCV-3 in Spain.
猪圆环病毒3型（PCV - 3）是圆环病毒科圆环病毒属的第三个成员，能够感染猪。在野猪中已记录到病毒DNA的高流行率。最近，在意大利野生反刍动物中鉴定出了PCV - 3 DNA。基于这些先前的结果，本研究评估了西班牙自由放养反刍动物和 物种中PCV - 3 DNA检测的频率。此外，还对PCV - 3 PCR阳性样本进行了基因特征分析。共采集了来自加泰罗尼亚（西班牙东北部）的801份血清样本，包括马鹿（；n = 108）、狍（；n = 87）、比利牛斯岩羚羊（；n = 133）、伊比利亚野山羊（；n = 92）、摩弗伦羊（；n = 91）、黇鹿（；n = 104）、欧洲兔（；n = 101）和欧洲野兔（；n = 85），通过常规聚合酶链反应（PCR）进行检测，阳性样本进行测序。总体而言，在801份分析血清中有3份（0.37%）检测到PCV - 3 DNA，分别对应1只马鹿（1/108，0.9%）、1只摩弗伦羊（1/91，1.1%）和1只黇鹿（1/104，0.96%）。从 物种采集的样本均未PCR阳性。在阳性样本中检测到的部分基因组序列与在野猪和家猪中检测到的一些PCV - 3序列具有高度同一性（分别为99.7%和100%）。总之，本研究表明自由放养的反刍动物和 物种在西班牙PCV - 3的流行病学中不具有相关性。
