三光子头佩戴显微镜，用于在自由活动的大鼠中成像深层皮层。
Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats.
机构信息
Department of Behavior and Brain Organization, Research Center Caesar, Bonn, Germany.
Max Planck Institute for the Science of Light, Erlangen, Germany.
出版信息
Nat Methods. 2020 May;17(5):509-513. doi: 10.1038/s41592-020-0817-9. Epub 2020 May 4.
We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1 h in freely moving rats performing a range of behaviors.
我们设计了一种头戴式三光子显微镜，用于在自由活动的大鼠中对深层皮质层神经元活动进行成像。在 1320nm 处传输高能激发脉冲既需要空心光纤，又需要其传输特性不随光纤移动而变化的光纤，还需要色散补偿。这些发展使我们能够在距离皮质表面 >1.1mm 深处进行成像，并使自由活动的大鼠在执行各种行为时稳定地对第 5 层神经元活动进行 >1 小时的成像。
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