Department of Behavior and Brain Organization, Research Center Caesar, Bonn, Germany.
Max Planck Institute for the Science of Light, Erlangen, Germany.
Nat Methods. 2020 May;17(5):509-513. doi: 10.1038/s41592-020-0817-9. Epub 2020 May 4.
We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1 h in freely moving rats performing a range of behaviors.
我们设计了一种头戴式三光子显微镜，用于在自由活动的大鼠中对深层皮质层神经元活动进行成像。在 1320nm 处传输高能激发脉冲既需要空心光纤，又需要其传输特性不随光纤移动而变化的光纤，还需要色散补偿。这些发展使我们能够在距离皮质表面 >1.1mm 深处进行成像，并使自由活动的大鼠在执行各种行为时稳定地对第 5 层神经元活动进行 >1 小时的成像。
