用于大视场、多平面和长期脑成像的微型双光子显微镜。
Miniature two-photon microscopy for enlarged field-of-view, multi-plane and long-term brain imaging.
机构信息
State Key Laboratory of Membrane Biology, Institute of Molecular Medicine, Peking-Tsinghua Center for Life Sciences, College of Future Technology, Peking University, Beijing, China.
Department of Cognitive Sciences, Institute of Basic Medical Sciences, Beijing, China.
出版信息
Nat Methods. 2021 Jan;18(1):46-49. doi: 10.1038/s41592-020-01024-z. Epub 2021 Jan 6.
We have developed a miniature two-photon microscope equipped with an axial scanning mechanism and a long-working-distance miniature objective to enable multi-plane imaging over a volume of 420 × 420 × 180 μm at a lateral resolution of ~1 μm. Together with the detachable design that permits long-term recurring imaging, our miniature two-photon microscope can help decipher neuronal mechanisms in freely behaving animals.
我们开发了一种配备轴向扫描机构和长工作距离微型物镜的微型双光子显微镜，可在 420×420×180μm 的体积上实现多平面成像，横向分辨率约为 1μm。结合可实现长期重复成像的可拆卸设计，我们的微型双光子显微镜可帮助破译自由活动动物中的神经元机制。
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