UMR 1332 Biologie du Fruit et Pathologie, INRAE, Univ. Bordeaux, 72 Avenue Edouard Bourlaux, CS20032, 33882, Villenave d'Ornon cedex, France.
UMR AGAP, INRAE, Montpellier SupAgro, TA A-108/03 Avenue Agropolis, 34398, Montpellier Cedex 5, France.
Arch Virol. 2020 Jul;165(7):1683-1685. doi: 10.1007/s00705-020-04640-4. Epub 2020 May 5.
Double-stranded RNAs and total RNAs purified from grapevine (Vitis vinifera) phloem scrapings of two varieties held in the INRAE (France) grapevine germplasm collection were analyzed by high-throughput sequencing. BLAST annotation revealed contigs with homology to Polerovirus genus members. The full genome sequence of one isolate (KT) was determined (5651 nucleotides [nt]), and a partial sequence representing about half of the genome was assembled for a second isolate (KS) that was found to share 95% nt sequence identity with the KT isolate. The genome has a typical polerovirus organization, containing six open reading frames (ORFs) as well as a putative additional ORF3a. Based on genome organization and phylogenetic relationships, the new virus belongs to the genus Polerovirus but, similar to the recently described persimmon polerovirus 1, is characterized by a highly divergent coat-protein/readthrough domain. Considering the species demarcation criteria for the family Luteoviridae, these two isolates, together with a closely related sequence recently deposited in the GenBank database (LC507098), represent a new Polerovirus species for which the name "Grapevine polerovirus 1" is proposed.
通过高通量测序分析了从法国 INRAE（法国）葡萄种质资源收集的两个品种的葡萄韧皮部刮屑中纯化的双链 RNA 和总 RNA。BLAST 注释揭示了与 Polerovirus 属成员具有同源性的序列。一个分离株（KT）的全长基因组序列已确定（5651 个核苷酸[nt]），并组装了第二个分离株（KS）的大约一半基因组的部分序列，发现该序列与 KT 分离株的 nt 序列同一性为 95%。基因组具有典型的杆状病毒结构，包含六个开放阅读框（ORF）以及一个假定的附加 ORF3a。基于基因组组织和系统发育关系，新病毒属于 Polerovirus 属，但与最近描述的柿子杆状病毒 1 相似，其外壳蛋白/通读结构域高度分化。考虑到 Luteoviridae 科的物种划分标准，这两个分离株以及最近在 GenBank 数据库中提交的密切相关序列（LC507098）代表了一种新的 Polerovirus 物种，建议将其命名为“葡萄杆状病毒 1”。
