China Center for Type Culture Collection (CCTCC), College of Life Sciences, Wuhan University, Wuhan 430072, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3455-3461. doi: 10.1099/ijsem.0.004194.
A Gram-stain-negative, rod-shaped, green-pigmented, aerobic and motile bacterium, strain R3-44, was isolated from Arctic tundra soil. Stain R3-44 clustered closely with members of the genus , which belongs to the family , and showed the highest 16S rRNA sequence similarity to AR2 (96.10%). Strain R3-44 grew optimally at pH 7.0, 28 °C and in the presence of 0-0.5 % (w/v) NaCl. The predominant respiratory isoprenoid quinone of strain R3-44 was identified as ubiquinone Q-8. The polar lipids consisted of phosphatidylglycerol, phosphatidylethanolamine, unidentified aminolipid and unidentified phospholipid. The main fatty acids were summed feature 3 (comprising C ω7 and/or C ω6, 40.6 %) and C (29.3 %). The DNA G+C content of strain R3-44 was 60.8 mol%. On the basis of the evidence presented in this study, strain R3-44 represents a novel species of the genus , for which the name sp. nov. is proposed, with the type strain R3-44 (=CCTCC AB 2010422=KCTC 72602).
一株革兰氏阴性、杆状、绿色、需氧和能动的细菌,菌株 R3-44,从北极苔原土壤中分离得到。R3-44 菌株与属内的成员聚类密切,与 AR2(96.10%)具有最高的 16S rRNA 序列相似性。R3-44 菌株在 pH 值为 7.0、28°C 和 0-0.5%(w/v)NaCl 存在下生长最佳。R3-44 菌株的主要呼吸异戊二烯醌是泛醌 Q-8。极性脂类由磷脂酰甘油、磷脂酰乙醇胺、未鉴定的胺脂和未鉴定的磷脂组成。主要脂肪酸为总和特征 3(包括 C ω7 和/或 C ω6,40.6%)和 C(29.3%)。R3-44 菌株的 DNA G+C 含量为 60.8mol%。基于本研究提供的证据,R3-44 菌株代表了属的一个新种,建议使用新种名 ,其模式菌株为 R3-44(=CCTCC AB 2010422=KCTC 72602)。