Guangxi Key Laboratory on the Study of Coral Reefs in the South China Sea, Nanning 530004, PR China.
School of Marine Sciences, Guangxi University, Nanning 530004, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3427-3432. doi: 10.1099/ijsem.0.004189.
A Gram-stain-negative, non-spore-forming, aerobic, curved rod-shaped bacterium, designed strain R142, was isolated from a coralline algae sp. in the Beibu Gulf, China. Optimal growth occurred with 0-0.5 % (w/v) NaCl, at 25 °C and at pH 8. Global alignment based on 16S rRNA gene sequences indicated that strain R142 shared 93.8 % similarity with its closest type strain, KU14G. Phylogenetic analyses showed that strain R142 forms a distinct branch alongside KU41E, SW-11, KU14G, MEBiC06469 and SM-6. The major polar lipids of strain R142 were phosphatidylethanolamine and phosphatidylglycerol. The primary cellular fatty acids were C, Cω7, Cω7, C and C. The genome DNA G+C ratio was 56.4 mol%. The only detected respiratory quinone was ubiquinone 8. The low 16S rRNA gene sequence similarity and differences in cellular fatty acids readily distinguished strain R142 from all validly published type strains. Strain R142 is therefore suggested to represent a novel species of a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain of is R142 (=MCCC 1K03816=KCTC 72138).
一株革兰氏阴性、不产芽孢、需氧、弯曲杆状细菌，设计菌株 R142，从中国北部湾的一种珊瑚藻中分离得到。最佳生长条件为 0-0.5%（w/v）NaCl、25°C 和 pH8。基于 16S rRNA 基因序列的全局比对表明，菌株 R142 与最接近的模式菌株 KU14G 具有 93.8%的相似性。系统发育分析表明，菌株 R142 与 KU41E、SW-11、KU14G、MEBiC06469 和 SM-6 一起形成一个独特的分支。菌株 R142 的主要极性脂是磷脂酰乙醇胺和磷脂酰甘油。主要的细胞脂肪酸是 C、Cω7、Cω7、C 和 C。基因组 DNA G+C 含量为 56.4mol%。唯一检测到的呼吸醌是泛醌 8。16S rRNA 基因序列的低相似性和细胞脂肪酸的差异很容易将菌株 R142 与所有已发表的有效模式菌株区分开来。因此，建议菌株 R142 代表一个新属的新种，建议使用新属名和新种名。其模式菌株为 R142（=MCCC 1K03816=KCTC 72138）。
