Marine College, Shandong University, Weihai, Shandong, 264209, PR China.
State key Laboratory of Microbial Technology, Shandong University, Qingdao, Shandong, 266237, PR China.
Int J Syst Evol Microbiol. 2021 Jan;71(1). doi: 10.1099/ijsem.0.004558. Epub 2020 Dec 17.
A novel Gram-stain-negative, strictly aerobic, coccoid and agar-hydrolysing bacterium, designated RQJ05, was isolated from the marine red algae collected from the coastal area of Rizhao, PR China. Cells of strain RQJ05 were approximately 0.8-1.0×1.3-3.0 µm in size and motile by means of a polar flagellum. Growth occurred at 4-33 °C (optimum, 25-30 °C), pH 7.0-8.5 (optimum, pH 7.5-8.0) and in the presence of 1.0-7.0 % (w/v) NaCl (optimum, 2.0-3.0 %). Strain RQJ05 showed oxidase-positive and catalase-negative activities. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain RQJ05 formed a phylogenetic lineage with members of the family and exhibited 16S rRNA gene sequence similarities of 92.6, 91.3, 90.2 and 90.1 % to Q1, YM01, NSCS20N07D and B-10-31, respectively. The major cellular fatty acids of strain RQJ05 were summed feature 3 (C 7 and/or C 6), C and summed feature 8 (C 7 and/or C 6). The major polar lipids of strain RQJ05 were phosphatidylethanolamine, phosphatidylglycerol and two aminophospholipids. Strain RQJ05 contained Q-8 as the major respiratory quinone. The genomic DNA G+C content was 39.0 mol%. On the basis of genotypic, phenotypic and phylogenetic evidence, strain RQJ05 is presented as a representative of a novel species in a new genus, for which the name gen. nov., sp. nov. is proposed. The type strain is RQJ05 (=KCTC 62846=MCCC 1H00352).
一株新型革兰氏阴性、严格需氧、球形且能水解琼脂的细菌，被命名为 RQJ05，从中国日照沿海地区采集的海洋红藻中分离得到。RQJ05 菌株的细胞大小约为 0.8-1.0×1.3-3.0μm，通过极生鞭毛运动。该菌在 4-33℃（最适温度 25-30℃）、pH7.0-8.5（最适 pH7.5-8.0）和 1.0-7.0%（w/v）NaCl（最适 2.0-3.0%）的条件下生长。RQJ05 菌株氧化酶阳性，过氧化氢酶阴性。基于 16S rRNA 基因序列的系统发育分析表明，RQJ05 菌株与属内的成员形成一个进化枝，与 Q1、YM01、NSCS20N07D 和 B-10-31 的 16S rRNA 基因序列相似性分别为 92.6%、91.3%、90.2%和 90.1%。RQJ05 菌株的主要细胞脂肪酸为 C 7 和/或 C 6 的总和特征 3、C 16:0 和 C 7 和/或 C 6 的总和特征 8。RQJ05 菌株的主要极性脂为磷脂酰乙醇胺、磷脂酰甘油和两种氨基磷脂。RQJ05 菌株含有 Q-8 作为主要的呼吸醌。基因组 DNA G+C 含量为 39.0mol%。基于基因型、表型和系统发育证据，RQJ05 菌株被提议代表一个新属的新种，命名为。模式菌株为 RQJ05（=KCTC 62846=MCCC 1H00352）。
