sp. nov., isolated from leaves of on the Qinghai-Tibet Plateau.

Two novel Gram-stain-positive, irregular rod-shaped actinomycetes, S-1144 and 4053, were isolated from leaves of on the Qinghai-Tibet Plateau, PR China. Cells were aerobic, catalase-positive and oxidase-negative. Colonies on Reasoner's 2A agar were light yellow, circular, shiny, smooth and convex after 2 days of incubation. The isolates grew optimally at 25 °C, pH 7.5 and with 0 % (w/v) NaCl. The results of polyphasic analyses indicated that strain S-1144 belonged to the genus and its close phylogenetic neighbours (16S rRNA gene sequence similarity) were DSM 103718 (98.4 %), DSM 23986 (98.2%) and DSM 11054 (97.8 %). The genome of strain S-1144 showed less than 70 % digital DNA-DNA hybridization and < 95-96 % average nucleotide identity values to the above reference strains. The DNA G+C content of strain S-1144 was 73.5 mol%. MK-8(H) was the predominant respiratory quinone (96.0 %) and llLL-2,6-diaminopimelic acid was the diagnostic diamino acid in the cell-wall peptidoglycan. The polar lipid profile of strain S-1144 consisted of diphosphatidylglycerol, phosphatidylglycerol, three unidentified phospholipids, one unidentified glycolipid and one unidentified lipid. The major cellular fatty acids were iso-C, C 8, C and C 9. On the basis of obtained data, strain S-1144 represented a novel species of the genus , for which the name sp. nov. is proposed. The type strain is S-1144 (=CGMCC 4.7568=JCM 33469).