Sevgi Büşra, Leblebici Sema
Institute of Graduate Education, Department of Molecular Biology and Genetics, Bilecik Şeyh Edebali University, Bilecik, 11230 Türkiye.
Faculty of Science, Department of Molecular Biology and Genetics, Bilecik Şeyh Edebali University, Bilecik, 11230 Türkiye.
Physiol Mol Biol Plants. 2025 Mar;31(3):405-418. doi: 10.1007/s12298-025-01571-9. Epub 2025 Mar 19.
Salinity, a major ecological problem worldwide, adversely affects plant growth and productivity. Osmoprotectants are a possible strategy for plants to cope with and regulate their response to unfavorable environmental conditions, such as salinity. However, the role of sucrose in this process requires more precise elucidation. This study aims to investigate the ameliorative role of sucrose on growth parameters, proline content, antioxidant enzyme activity, and gene expression in sunflower and canola under salt stress. The treatments included a 3% sucrose concentration and two levels of salinity (75 and 150 mM NaCl). Salinity caused a remarkable reduction in stem-root growth, chlorophyll amounts and catalase (CAT) activity, whereas it unchanged ascorbate peroxidase (APX) activity. Furthermore, both plants grown under salt stress had considerably higher total protein, proline, malondialdehyde (MDA) content, and superoxide dismutase (SOD) activity. Exogenous sucrose increased plant growth, chlorophyll amounts and the activities of hydrogen peroxide-detoxifying antioxidant enzymes such as CAT and APX in salt-stressed plants, but dramatically depressed levels of osmoregulators such as protein and proline. Besides that, it balanced antioxidant enzyme levels by regulating SOD activity to the required level, thereby facilitating the effective operation of the antioxidant defense system. Additionally, sucrose had a different effect on gene expressions of antioxidants in sunflower and canola under salinity. These results revealed that sucrose can ameliorate the deleterious effects of salinity in sunflower and canola by modulating osmotic substance accumulation, the activity of antioxidant enzymes, and their gene expression. In conclusion, sucrose can be a potential tool for plants in salt stress alleviation.
The online version contains supplementary material available at 10.1007/s12298-025-01571-9.
盐度是全球主要的生态问题，对植物生长和生产力产生不利影响。渗透保护剂是植物应对和调节其对不利环境条件（如盐度）反应的一种可能策略。然而，蔗糖在此过程中的作用需要更精确的阐明。本研究旨在探讨蔗糖对盐胁迫下向日葵和油菜生长参数、脯氨酸含量、抗氧化酶活性及基因表达的改善作用。处理包括3%的蔗糖浓度和两个盐度水平（75和150 mM NaCl）。盐度导致茎根生长、叶绿素含量和过氧化氢酶（CAT）活性显著降低，而抗坏血酸过氧化物酶（APX）活性未发生变化。此外，在盐胁迫下生长的两种植物的总蛋白、脯氨酸、丙二醛（MDA）含量和超氧化物歧化酶（SOD）活性均显著更高。外源蔗糖增加了盐胁迫植物的生长、叶绿素含量以及过氧化氢解毒抗氧化酶如CAT和APX的活性，但显著降低了蛋白质和脯氨酸等渗透调节剂的水平。除此之外，它通过将SOD活性调节到所需水平来平衡抗氧化酶水平，从而促进抗氧化防御系统的有效运行。此外，蔗糖对盐度下向日葵和油菜中抗氧化剂的基因表达有不同影响。这些结果表明，蔗糖可以通过调节渗透物质积累、抗氧化酶活性及其基因表达来改善盐度对向日葵和油菜的有害影响。总之，蔗糖可能是植物缓解盐胁迫的一种潜在工具。
在线版本包含可在10.1007/s12298-025-01571-9获取的补充材料。
