Angouti Farinaz, Nourafcan Hassan, Saeedi Sar Sakineh, Assadi Assad, Ebrahimi Raheleh
Department of Horticultural Science and Agronomy, Science and Research Branch Islamic Azad University Tehran Iran.
Department of Horticulture, Medicinal Plants and Organic Products Research Center, Miyaneh Branch Islamic Azad University Miyaneh Iran.
Food Sci Nutr. 2024 May 27;12(8):5844-5857. doi: 10.1002/fsn3.4204. eCollection 2024 Aug.
Diabetes poses a significant global health burden, demanding safe and effective therapeutic interventions. Medicinal plants offer promising avenues for natural diabetic management. (goat's rue) has long been recognized for its hypoglycemic potential, but optimizing its phytochemical content and antidiabetic activity remains a key challenge. This study aimed to address this aspect by investigating the impact of foliar application of chitosan and salicylic acid on the physiological and phytochemical properties of , and subsequently evaluating its antidiabetic efficacy compared to that of the established drug metformin. A randomized complete block design with three replications was employed. Laboratory mice were divided into treatment groups receiving extract from plants sprayed with four salicylic acid concentrations (0.5-3 mM/L) and four chitosan concentrations (0-0.8 g/L). Blood glucose levels and various physiological parameters were assessed. Chitosan at 0.4 g/L and salicylic acid at 2 mM significantly enhanced the growth, photosynthetic pigments, and antioxidant activity of . Notably, the extract from plants treated with 3 mM salicylic acid exhibited the highest total alkaloid content, a potential contributor to antidiabetic activity. In a separate study, diabetic mice treated with this optimized extract (50 mg/kg) exhibited significantly greater blood glucose reductions compared to those treated with metformin (500 mg). This study demonstrates the potential of chitosan and salicylic acid in optimizing the beneficial properties of . The extract derived from plants treated with 3 mM salicylic acid displayed superior blood glucose-lowering efficacy compared to metformin, suggesting its promising role as a potential natural antidiabetic therapy. Further research is warranted to elucidate the specific bioactive compounds responsible for this enhanced activity and translate these findings into clinical applications.
糖尿病给全球带来了重大的健康负担，需要安全有效的治疗干预措施。药用植物为糖尿病的自然管理提供了有前景的途径。长期以来，山羊豆因其降血糖潜力而受到认可，但优化其植物化学成分和抗糖尿病活性仍然是一项关键挑战。本研究旨在通过研究壳聚糖和水杨酸叶面喷施对山羊豆生理和植物化学特性的影响来解决这一问题，并随后与已确立的药物二甲双胍相比评估其抗糖尿病疗效。采用了具有三次重复的随机完全区组设计。将实验室小鼠分为治疗组，接受用四种水杨酸浓度（0.5 - 3 mM/L）和四种壳聚糖浓度（0 - 0.8 g/L）喷施的植物提取物。评估血糖水平和各种生理参数。0.4 g/L的壳聚糖和2 mM的水杨酸显著提高了山羊豆的生长、光合色素和抗氧化活性。值得注意的是，用3 mM水杨酸处理的植物提取物显示出最高的总生物碱含量，这可能是抗糖尿病活性的一个促成因素。在另一项研究中，与用二甲双胍（500 mg）治疗的糖尿病小鼠相比，用这种优化的山羊豆提取物（50 mg/kg）治疗的小鼠血糖降低更为显著。本研究证明了壳聚糖和水杨酸在优化山羊豆有益特性方面的潜力。与二甲双胍相比，用3 mM水杨酸处理的植物提取物显示出更高的降血糖功效，表明其作为潜在的天然抗糖尿病疗法具有广阔前景。有必要进一步研究以阐明导致这种增强活性的具体生物活性化合物，并将这些发现转化为临床应用。
