Laboratorio de Diabetes y Toxicología de la Reproducción-Teratogénesis, Departamento de Farmacia, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Av. Wilfrido Massieu S/N, Unidad Adolfo López Mateos, Mexico City, Mexico.
Lab. Investigación Biomédica en Productos Naturales, Carrera Medicina, FES Iztacala, UNAM, Avenida de los Barrios Número 1, Colonia Los Reyes Iztacala, Estado de México, C.P, Tlalnepantla, 54090, Mexico.
Mediators Inflamm. 2020 Apr 21;2020:5062506. doi: 10.1155/2020/5062506. eCollection 2020.
is a plant used in traditional Mexican medicine to treat different gastrointestinal problems. Here, we investigated the effects of a methanolic extract in DSS-induced colitis mice. Ulcerative colitis (UC) was induced by administering 4% DSS in drinking water to female BALB/c mice. Compared to untreated mice with UC, the treatment group receiving the extract presented less severe UC symptoms of diarrhea, bleeding, and weight loss. Additionally, colon shortening was significantly reduced, and at the microscopic level, only minor damage was observed. Levels of proinflammatory cytokines such as TNF-, IL-1, and IFN in serum as well as the MPO activity in the colon were significantly reduced in the methanolic extract-treated group. Moreover, the extract of reduced oxidative stress during UC, preventing the deterioration of the activity of antioxidant enzymes such as SOD, CAT, and GPx. Additionally, the extract decreased lipid peroxidation damage and its final products, such as malondialdehyde (MDA). In agreement with this, assays with the extract displayed good antioxidant capacity, probably due to the presence of polyphenolic compounds, in particular the flavonoids that were identified, such as chrysin, naringenin, kaempferol, and catechin, which have been reported to have anti-inflammatory and antioxidant activities. Therefore, the improvement of UC by the methanolic extract may be related to the action mechanisms of these compounds.
是一种用于治疗不同胃肠道问题的传统墨西哥药物。在这里，我们研究了一种甲醇提取物对 DSS 诱导的结肠炎小鼠的影响。溃疡性结肠炎 (UC) 通过在饮用水中给予 4% DSS 诱导雌性 BALB/c 小鼠。与未治疗的 UC 小鼠相比，接受提取物治疗的小鼠的 UC 症状腹泻、出血和体重减轻明显减轻。此外，结肠缩短明显减少，在显微镜下仅观察到轻微损伤。血清中促炎细胞因子如 TNF-、IL-1 和 IFN 以及结肠中的 MPO 活性在甲醇提取物治疗组中显著降低。此外，在 UC 期间，提取物减少了氧化应激，防止了抗氧化酶如 SOD、CAT 和 GPx 的活性恶化。此外，提取物减少了脂质过氧化损伤及其终产物，如丙二醛 (MDA)。与此一致的是，与提取物的测定显示出良好的抗氧化能力，可能是由于存在多酚化合物，特别是已报道具有抗炎和抗氧化活性的类黄酮，如白杨素、柚皮素、山奈酚和儿茶素。因此，甲醇提取物对 UC 的改善可能与这些化合物的作用机制有关。
