菠萝（(L.) Merr.）中DREB转录因子家族的鉴定与表达分析。

Identification and expression analysis of the DREB transcription factor family in pineapple ( (L.) Merr.).

作者信息

Chai Mengnan, Cheng Han, Yan Maokai, Priyadarshani Svgn, Zhang Man, He Qing, Huang Youmei, Chen Fangqian, Liu Liping, Huang Xiaoyi, Lai Linyi, Chen Huihuang, Cai Hanyang, Qin Yuan

机构信息

State Key Lab of Ecological Pest Control for Fujian and Taiwan Crops; Key Lab of Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education; Fujian Provincial Key Lab of Haixia Applied Plant Systems Biology, Fujian Agriculture and Forestry University, Fuzhou, Fujian Province, China.

State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi Key Lab of Sugarcane Biology, College of Agriculture, Guangxi University, Nanning, Guangxi Province, China.

出版信息

PeerJ. 2020 Apr 28;8:e9006. doi: 10.7717/peerj.9006. eCollection 2020.

BACKGROUND

Dehydration responsive element-binding (DREB) transcription factors play a crucial role in plant growth, development and stress responses. Although genes have been characterized in many plant species, genome-wide identification of the gene family has not yet been reported in pineapple ( (L.) Merr.).

RESULTS

Using comprehensive genome-wide screening, we identified 20 genes on 14 chromosomes. These were categorized into five subgroups. within a group had similar gene structures and domain compositions. Using gene structure analysis, we showed that most genes (75%) lacked introns, and that the promoter regions of all 20 genes had at least one stress response-related -element. We identified four genes with high expression levels and six genes with low expression levels in all analyzed tissues. We detected expression changes under abiotic stress for eight selected genes.

CONCLUSIONS

This report presents the first genome-wide analysis of the DREB transcription factor family in pineapple. Our results provide preliminary data for future functional analysis of genes in pineapple, and useful information for developing new pineapple varieties with key agronomic traits such as stress tolerance.

背景

脱水响应元件结合（DREB）转录因子在植物生长、发育和胁迫响应中起关键作用。尽管在许多植物物种中已对该基因进行了表征，但尚未见菠萝（凤梨（L.）梅里尔）中该基因家族全基因组鉴定的报道。

结果

通过全面的全基因组筛选，我们在14条染色体上鉴定出20个DREB基因。这些基因被分为五个亚组。同一亚组内的基因具有相似的基因结构和结构域组成。通过基因结构分析，我们发现大多数DREB基因（75%）没有内含子，并且所有20个DREB基因的启动子区域至少有一个与胁迫响应相关的顺式作用元件。我们在所有分析的组织中鉴定出4个高表达基因和6个低表达基因。我们检测了8个选定DREB基因在非生物胁迫下的表达变化。