Genome-Wide Identification and Expression Profiling of CBL-CIPK Gene Family in Pineapple () and the Role of CBL1 in Abiotic and Biotic Stress Response.

Ca serves as a ubiquitous second messenger regulating several aspects of plant growth and development. A group of unique calcium sensor proteins, calcineurin B-like (CBL), interact with CBL-interacting protein kinases (CIPKs) to decode the Ca signature inside the cell. Although CBL-CIPK signaling toolkit has been shown to play significant roles in the responses to numerous stresses in different plants, the information about pineapple CBL-CIPK remains obscure. In the present study, a total of eight CBL and 21 CIPK genes were identified genome-wide in pineapple. The identified genes were renamed on the basis of gene ID in ascending order and phylogenetic analysis divided into five groups. Transcriptomic data analysis showed that CBL and CIPK genes were expressed differentially in different tissues. Further, the expression analysis of CBL1 in different tissues showed significant changes under various abiotic stimuli. Additionally, the ectopic expression of CBL1 in resulted in enhanced tolerance to salinity, osmotic, and fungal stress. The present study revealed the crucial contribution of the CBL-CIPK gene in various biological and physiological processes in pineapple.