Laboratory of Biotechnology, Dalian Institute of Chemical Physics, CAS, 457 Zhongshan Road, Dalian, 116023, China.
University of Chinese Academy of Sciences, 19 Yuquan Lu, Beijing, 100049, China.
Appl Biochem Biotechnol. 2020 Sep;192(1):283-295. doi: 10.1007/s12010-020-03331-9. Epub 2020 May 6.
The lipids produced by oleaginous microbes are considered sustainable resources for biofuels. To facilitate controlled lipid production and lipid analysis, more efficient lipid extraction methods are required. This study describes the automated pressurized liquid extraction (APLE) method for lipid extraction from dried cells of the oleaginous yeast species Rhodosporidium toruloides and Cryptococcus curvatus. Cells were mixed with diatomite in a mortar, added to the sample chamber, and treated with a mixture of chloroform and methanol at 105 °C. More than 95% lipids were extracted. Analysis by using high-performance thin-layer chromatography showed that the neutral lipid contents in the obtained samples by APLE method were similar to those by the ball milling-assisted extraction method. The lipids had an essentially identical fatty acid composition compared with lipids extracted with the acid-heating extraction (AHE) method. This demonstrated that lipids can be efficiently extracted from oleaginous yeasts in less time and without harsh pretreatment procedures.
油脂微生物产生的脂质被认为是生物燃料的可持续资源。为了便于控制脂质生产和脂质分析，需要更有效的脂质提取方法。本研究描述了从产油酵母属 Rhodosporidium toruloides 和隐球菌属 Cryptococcus curvatus 的干燥细胞中提取脂质的自动化加压液体萃取（APLE）方法。将细胞与硅藻土在研钵中混合，加入样品室，并用氯仿和甲醇混合物在 105°C 下处理。超过 95%的脂质被提取。通过高效薄层层析分析表明，APLE 法获得的样品中的中性脂质含量与球磨辅助提取法相似。与酸加热提取（AHE）法提取的脂质相比，这些脂质的脂肪酸组成基本相同。这表明可以在更短的时间内，无需苛刻的预处理程序，从油脂酵母中有效地提取脂质。
