Department of Fiber and Polymer Technology, KTH Royal Institute of Technology, Teknikringen 56-58, 100 44 Stockholm, Sweden.
Department of Micro and Nanosystems, KTH Royal Institute of Technology, Malvinas väg 10, 100 44 Stockholm, Sweden.
J Colloid Interface Sci. 2020 Sep 1;575:286-297. doi: 10.1016/j.jcis.2020.04.107. Epub 2020 Apr 27.
Antimicrobial surfaces are important in medical, clinical, and industrial applications, where bacterial infection and biofouling may constitute a serious threat to human health. Conventional approaches against bacteria involve coating the surface with antibiotics, cytotoxic polymers, or metal particles. However, these types of functionalization have a limited lifetime and pose concerns in terms of leaching and degradation of the coating. Thus, there is a great interest in developing long-lasting and non-leaching bactericidal surfaces. To obtain a bactericidal surface, we combine micro and nanoscale patterning of borosilicate glass surfaces by ultrashort pulsed laser irradiation and a non-leaching layer-by-layer polyelectrolyte modification of the surface. The combination of surface structure and surface charge results in an enhanced bactericidal effect against both Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli bacteria. The laser patterning and the layer-by-layer modification are environmentally friendly processes that are applicable to a wide variety of materials, which makes this method uniquely suited for fundamental studies of bacteria-surface interactions and paves the way for its applications in a variety of fields, such as in hygiene products and medical devices.
抗菌表面在医学、临床和工业应用中非常重要，因为细菌感染和生物污垢可能对人类健康构成严重威胁。传统的抗菌方法包括在表面涂覆抗生素、细胞毒性聚合物或金属颗粒。然而，这些类型的功能化具有有限的寿命，并存在涂层浸出和降解的问题。因此，人们非常感兴趣开发持久且无浸出的杀菌表面。为了获得杀菌表面，我们通过超短脉冲激光辐照对硼硅酸盐玻璃表面进行微纳尺度图案化，并对表面进行无浸出的层层聚电解质修饰。表面结构和表面电荷的组合对革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌细菌产生了增强的杀菌效果。激光图案化和层层修饰是环保的过程，适用于多种材料，这使得这种方法非常适合细菌-表面相互作用的基础研究，并为其在各种领域的应用铺平了道路，例如在卫生产品和医疗器械中。
