Zhang Fanjun, Hu Cheng, Yang Li, Liu Kunpeng, Ge Yao, Wei Yuan, Wang Jingyu, Luo Rifang, Wang Yunbing
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
J Mater Chem B. 2021 Mar 21;9(11):2697-2708. doi: 10.1039/d1tb00021g. Epub 2021 Mar 8.
Hospital-acquired infections and thrombosis caused by bacteria attached to the device surface, or fibrin crosslinking owing to platelet accumulation/activation, are major healthcare challenges that cause morbidity and mortality. To prevent these, surface coating technologies are considered an efficient tool that can combine hemocompatibility and bactericidal activity. In this study, surface-initiated polymerization was conducted to form an all-in-one hydrogel coating that could adapt to diverse medical devices. Different monomer ratios (acrylamide/acrylic acid) were used to adjust the antimicrobial agent loading capacity. The hydrogel coating obtained by a simple dip-absorbing method showed good hemocompatibility and maintained efficient bactericidal activity. We also explored the loading and release of antimicrobial agents with different molecular sizes, including nano-Ag particles, antibiotics, and antimicrobial peptides. The inhibition zone test and confocal laser scanning microscopy revealed that the hydrogel coating could maintain remarkable antimicrobial and antifouling properties for four weeks. Furthermore, the hydrogel coating decreased the platelet adhesion/activation without risk of hemolysis. The ex vivo blood circulation study confirmed the antithrombotic properties of the hydrogel coating. Such all-in-one hydrogel coatings that maintain high cell viability and exhibit both hemocompatibility and bactericidal activity possess the potential for applications in blood-contacting devices.
由附着在器械表面的细菌引起的医院获得性感染和血栓形成，或因血小板聚集/活化导致的纤维蛋白交联，是造成发病和死亡的主要医疗挑战。为预防这些问题，表面涂层技术被视为一种能够兼具血液相容性和杀菌活性的有效工具。在本研究中，进行了表面引发聚合反应以形成一种可适应多种医疗器械的一体化水凝胶涂层。使用不同的单体比例（丙烯酰胺/丙烯酸）来调节抗菌剂负载量。通过简单的浸吸法获得的水凝胶涂层表现出良好的血液相容性，并保持了高效的杀菌活性。我们还研究了不同分子大小的抗菌剂的负载和释放情况，包括纳米银颗粒、抗生素和抗菌肽。抑菌圈试验和共聚焦激光扫描显微镜显示，水凝胶涂层能够在四周内保持显著的抗菌和抗污性能。此外，水凝胶涂层降低了血小板的粘附/活化，且无溶血风险。体外血液循环研究证实了水凝胶涂层的抗血栓性能。这种保持高细胞活力并兼具血液相容性和杀菌活性的一体化水凝胶涂层在血液接触器械中具有应用潜力。
