Center for Perinatal Research, Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, OH, USA.
Department of Pediatrics, Ohio State University, Columbus, OH, USA.
Pediatr Res. 2021 Mar;89(4):889-893. doi: 10.1038/s41390-020-0943-1. Epub 2020 May 9.
Inflammation is strongly associated with premature birth and neonatal morbidities. Increases in infant haptoglobin, haptoglobin-related protein (Hp&HpRP), and interleukin-6 (IL-6) levels are indicators of intra-amniotic inflammation (IAI) and have been linked to poor neonatal outcomes. Inflammation causes epigenetic changes, specifically suppression of miR-29 expression. The current study sought to determine whether miR-29b levels in cord blood or neonatal venous blood are associated with IAI, identified by elevated IL-6 and Hp, and subsequent clinical morbidities in the infant.
We tested 92 cord blood samples from premature newborns and 18 venous blood samples at 36 weeks corrected gestational age. MiR-29b, Hp&HpRP, and IL-6 were measured by polymerase chain reaction and enzyme-linked immunosorbent assay, respectively.
Decreased levels of miR-29b were observed in infants exposed to IAI with elevated Hp&HpRP and IL-6 levels and in infants delivered by spontaneous preterm birth. Lower miR-29 levels were also observed in women diagnosed with histological chorioamnionitis or funisitis and in infants with cerebral palsy. Higher levels of miR-29 were measured in infants small for gestational age and in venous samples from older infants.
MiR-29 may be an additional biomarker of IAI and a potential therapeutic target for treating poor newborn outcomes resulting from antenatal exposure to IAI.
Decreases in miR-29b are associated with intrauterine inflammation. Hp&HpRP increases are associated with decreased miR-29b. MiR-29b may be an additional biomarker for neonatal outcomes and a potential therapeutic target for intrauterine inflammation.
炎症与早产和新生儿病率密切相关。婴儿结合珠蛋白、结合珠蛋白相关蛋白（Hp&HpRP）和白细胞介素-6（IL-6）水平的升高是羊膜内炎症（IAI）的指标，并与新生儿不良结局相关。炎症会导致表观遗传变化，特别是 miR-29 表达的抑制。本研究旨在确定脐带血或新生儿静脉血中的 miR-29b 水平是否与通过升高的 IL-6 和 Hp 识别的 IAI 以及婴儿随后的临床病率相关。
我们检测了 92 例早产新生儿的脐带血样本和 18 例 36 周校正胎龄的静脉血样本。通过聚合酶链反应和酶联免疫吸附试验分别检测 miR-29b、Hp&HpRP 和 IL-6。
在暴露于升高的 Hp&HpRP 和 IL-6 水平的 IAI 婴儿以及自发性早产分娩的婴儿中观察到 miR-29b 水平降低。在诊断为组织学绒毛膜炎或脐带炎的妇女和脑瘫婴儿中也观察到 miR-29 水平较低。在小于胎龄儿的婴儿中测量到较高水平的 miR-29，在较大婴儿的静脉样本中也测量到较高水平的 miR-29。
miR-29 可能是 IAI 的另一个生物标志物，也是治疗因产前暴露于 IAI 而导致的新生儿不良结局的潜在治疗靶点。
miR-29b 的减少与宫内炎症有关。Hp&HpRP 的增加与 miR-29b 的减少有关。miR-29b 可能是新生儿结局的另一个生物标志物，也是宫内炎症的潜在治疗靶点。
